Characterization of the universal stress protein F from atypical enteropathogenic Escherichia coli and its prevalence in Enterobacteriaceae

Cristiane S. de Souza, Alfredo Torres, Andressa Caravelli, Anderson Silva, Juliana M. Polatto, Roxane M F Piazza

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Atypical enteropathogenic Escherichia coli (aEPEC) are heterogeneous strains in terms of serotypes, adherence patterns and the presence of novel virulence factors. This heterogeneity is intriguing, promoting studies trying to characterize these novel proteins and to better comprehend this pathotype group. In a previous study analyzing low-molecular mass proteomes of four representative aEPEC strains of three different adhesion phenotypes, we classified proteins according to their annotated function, with most of them being involved in metabolism and transport; while some of them were classified as hypothetical proteins. The majority of the hypothetical proteins were homologue products of genes identified in the genome of enterohemorrhagic E. coli. One of the hypothetical proteins was annotated as Z2335, with orthologue in EPEC, and by bioinformatics analysis, this protein was revealed to be the universal stress protein F (UspF). Thus, herein we successfully obtained a recombinant UspF protein from aEPEC, which is a α/β, ATP-binding protein involved in stress response, with comparable protein production among the four studied strains, but showing noteworthy differences when cultivated in different stress conditions, also present in other enterobacterial species, such as Shigella sonnei and Citrobacter freundii. Furthermore, our results confirm that the Usp protein superfamily encompasses a conserved group of proteins involved in stress resistance in aEPEC and other Enterobacteriaceae.

Original languageEnglish (US)
Pages (from-to)2142-2151
Number of pages10
JournalProtein Science
Volume25
Issue number12
DOIs
StatePublished - Dec 1 2016

Fingerprint

Enteropathogenic Escherichia coli
Enterobacteriaceae
Heat-Shock Proteins
Escherichia coli
Proteins
Genes
Shigella sonnei
Citrobacter freundii
Enterohemorrhagic Escherichia coli
Molecular mass
Virulence Factors
Proteome
Bioinformatics
Computational Biology
Metabolism
Carrier Proteins
Adhesion
Adenosine Triphosphate
Genome
Phenotype

Keywords

  • atypical EPEC
  • cloning
  • expression
  • hypothetical proteins
  • stress
  • UspF protein
  • α/β protein

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Characterization of the universal stress protein F from atypical enteropathogenic Escherichia coli and its prevalence in Enterobacteriaceae. / de Souza, Cristiane S.; Torres, Alfredo; Caravelli, Andressa; Silva, Anderson; Polatto, Juliana M.; Piazza, Roxane M F.

In: Protein Science, Vol. 25, No. 12, 01.12.2016, p. 2142-2151.

Research output: Contribution to journalArticle

de Souza, Cristiane S. ; Torres, Alfredo ; Caravelli, Andressa ; Silva, Anderson ; Polatto, Juliana M. ; Piazza, Roxane M F. / Characterization of the universal stress protein F from atypical enteropathogenic Escherichia coli and its prevalence in Enterobacteriaceae. In: Protein Science. 2016 ; Vol. 25, No. 12. pp. 2142-2151.
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