Characterization of the yehUT two-component regulatory system of Salmonella enterica serovar Typhi and Typhimurium

Vanessa K. Wong, Derek J. Pickard, Lars Barquist, Karthikeyan Sivaraman, Andrew J. Page, Peter J. Hart, Mark J. Arends, Kathryn E. Holt, Leanne Kane, Lynda F. Mottram, Louise Ellison, Ruben Bautista, Chris J. McGee, Sally J. Kay, Thomas M. Wileman, Linda Kenney, Calman A. MacLennan, Robert A. Kingsley, Gordon Dougan

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Proteins exhibiting hyper-variable sequences within a bacterial pathogen may be associated with host adaptation. Several lineages of the monophyletic pathogen Salmonella enterica serovar Typhi (S. Typhi) have accumulated non-synonymous mutations in the putative two-component regulatory system yehUT. Consequently we evaluated the function of yehUT in S. Typhi BRD948 and S. Typhimurium ST4/74. Transcriptome analysis identified the cstA gene, encoding a carbon starvation protein as the predominantly yehUT regulated gene in both these serovars. Deletion of yehUT had no detectable effect on the ability of these mutant Salmonella to invade cultured epithelial cells (S. Typhi and S. Typhimurium) or induce colitis in a murine model (S. Typhimurium only). Growth, metabolic and antimicrobial susceptibility tests identified no obvious influences of yehUT on these phenotypes.

Original languageEnglish (US)
Article numbere84567
JournalPloS one
Volume8
Issue number12
DOIs
StatePublished - Dec 30 2013
Externally publishedYes

Fingerprint

Salmonella Typhi
Salmonella typhi
Salmonella
Pathogens
Salmonella Typhimurium
serotypes
Gene encoding
pathogens
colitis
Gene Expression Profiling
Colitis
Starvation
transcriptomics
cultured cells
Genes
starvation
Cultured Cells
Proteins
epithelial cells
Carbon

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Wong, V. K., Pickard, D. J., Barquist, L., Sivaraman, K., Page, A. J., Hart, P. J., ... Dougan, G. (2013). Characterization of the yehUT two-component regulatory system of Salmonella enterica serovar Typhi and Typhimurium. PloS one, 8(12), [e84567]. https://doi.org/10.1371/journal.pone.0084567

Characterization of the yehUT two-component regulatory system of Salmonella enterica serovar Typhi and Typhimurium. / Wong, Vanessa K.; Pickard, Derek J.; Barquist, Lars; Sivaraman, Karthikeyan; Page, Andrew J.; Hart, Peter J.; Arends, Mark J.; Holt, Kathryn E.; Kane, Leanne; Mottram, Lynda F.; Ellison, Louise; Bautista, Ruben; McGee, Chris J.; Kay, Sally J.; Wileman, Thomas M.; Kenney, Linda; MacLennan, Calman A.; Kingsley, Robert A.; Dougan, Gordon.

In: PloS one, Vol. 8, No. 12, e84567, 30.12.2013.

Research output: Contribution to journalArticle

Wong, VK, Pickard, DJ, Barquist, L, Sivaraman, K, Page, AJ, Hart, PJ, Arends, MJ, Holt, KE, Kane, L, Mottram, LF, Ellison, L, Bautista, R, McGee, CJ, Kay, SJ, Wileman, TM, Kenney, L, MacLennan, CA, Kingsley, RA & Dougan, G 2013, 'Characterization of the yehUT two-component regulatory system of Salmonella enterica serovar Typhi and Typhimurium', PloS one, vol. 8, no. 12, e84567. https://doi.org/10.1371/journal.pone.0084567
Wong, Vanessa K. ; Pickard, Derek J. ; Barquist, Lars ; Sivaraman, Karthikeyan ; Page, Andrew J. ; Hart, Peter J. ; Arends, Mark J. ; Holt, Kathryn E. ; Kane, Leanne ; Mottram, Lynda F. ; Ellison, Louise ; Bautista, Ruben ; McGee, Chris J. ; Kay, Sally J. ; Wileman, Thomas M. ; Kenney, Linda ; MacLennan, Calman A. ; Kingsley, Robert A. ; Dougan, Gordon. / Characterization of the yehUT two-component regulatory system of Salmonella enterica serovar Typhi and Typhimurium. In: PloS one. 2013 ; Vol. 8, No. 12.
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