Characterizing recombinant chicken Thy-1 with reduced glycoform distribution by MALDI-TOF and ESI FT/ICR MS of AspN digested glycoprotein

Promod Mehndiratta, Silvia M. Pulido, Wendy J. Walton, Mark Emmett, Joan Hare, Alan G. Marshall, M. Timothy Logan

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

A method was developed to enhance the carbohydrate homogeneity of recombinant protein. The secreted protein was purified using a combination of ultrafiltration, lectin affinity chromatography, ion exchange chromatography and reverse-phase HPLC. The disulphide linkages and the glycoform distribution on all three glycosylation sites were characterized by combining N-terminal sequencing with matrix-assisted laser desorption ionization time of flight (MALDI-TOF) and ESI FT-ICR MS. The techniques confirmed the expected disulphide linkage pattern.

Original languageEnglish (US)
Title of host publicationProceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics
Pages467-468
Number of pages2
StatePublished - 2002
Externally publishedYes
EventProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States
Duration: Jun 2 2002Jun 6 2002

Other

OtherProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
CountryUnited States
CityOrlando, FL
Period6/2/026/6/02

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Disulfides
Ionization
Desorption
Glycoproteins
Glycosylation
Affinity chromatography
Lasers
Ultrafiltration
Chromatography
Recombinant Proteins
Lectins
Ion exchange
Carbohydrates
Proteins

ASJC Scopus subject areas

  • Spectroscopy

Cite this

Mehndiratta, P., Pulido, S. M., Walton, W. J., Emmett, M., Hare, J., Marshall, A. G., & Logan, M. T. (2002). Characterizing recombinant chicken Thy-1 with reduced glycoform distribution by MALDI-TOF and ESI FT/ICR MS of AspN digested glycoprotein. In Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics (pp. 467-468)

Characterizing recombinant chicken Thy-1 with reduced glycoform distribution by MALDI-TOF and ESI FT/ICR MS of AspN digested glycoprotein. / Mehndiratta, Promod; Pulido, Silvia M.; Walton, Wendy J.; Emmett, Mark; Hare, Joan; Marshall, Alan G.; Logan, M. Timothy.

Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. 2002. p. 467-468.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Mehndiratta, P, Pulido, SM, Walton, WJ, Emmett, M, Hare, J, Marshall, AG & Logan, MT 2002, Characterizing recombinant chicken Thy-1 with reduced glycoform distribution by MALDI-TOF and ESI FT/ICR MS of AspN digested glycoprotein. in Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. pp. 467-468, Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States, 6/2/02.
Mehndiratta P, Pulido SM, Walton WJ, Emmett M, Hare J, Marshall AG et al. Characterizing recombinant chicken Thy-1 with reduced glycoform distribution by MALDI-TOF and ESI FT/ICR MS of AspN digested glycoprotein. In Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. 2002. p. 467-468
Mehndiratta, Promod ; Pulido, Silvia M. ; Walton, Wendy J. ; Emmett, Mark ; Hare, Joan ; Marshall, Alan G. ; Logan, M. Timothy. / Characterizing recombinant chicken Thy-1 with reduced glycoform distribution by MALDI-TOF and ESI FT/ICR MS of AspN digested glycoprotein. Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. 2002. pp. 467-468
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