Characterizing recombinant chicken Thy-1 with reduced glycoform distribution by MALDI-TOF and ESI FT/ICR MS of AspN digested glycoprotein

Promod Mehndiratta, Silvia M. Pulido, Wendy J. Walton, Mark R. Emmett, Joan Hare, Alan G. Marshall, M. Timothy Logan

Research output: Contribution to conferencePaper

Abstract

A method was developed to enhance the carbohydrate homogeneity of recombinant protein. The secreted protein was purified using a combination of ultrafiltration, lectin affinity chromatography, ion exchange chromatography and reverse-phase HPLC. The disulphide linkages and the glycoform distribution on all three glycosylation sites were characterized by combining N-terminal sequencing with matrix-assisted laser desorption ionization time of flight (MALDI-TOF) and ESI FT-ICR MS. The techniques confirmed the expected disulphide linkage pattern.

Original languageEnglish (US)
Pages467-468
Number of pages2
StatePublished - Dec 1 2002
Externally publishedYes
EventProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States
Duration: Jun 2 2002Jun 6 2002

Other

OtherProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
CountryUnited States
CityOrlando, FL
Period6/2/026/6/02

ASJC Scopus subject areas

  • Spectroscopy

Fingerprint Dive into the research topics of 'Characterizing recombinant chicken Thy-1 with reduced glycoform distribution by MALDI-TOF and ESI FT/ICR MS of AspN digested glycoprotein'. Together they form a unique fingerprint.

  • Cite this

    Mehndiratta, P., Pulido, S. M., Walton, W. J., Emmett, M. R., Hare, J., Marshall, A. G., & Logan, M. T. (2002). Characterizing recombinant chicken Thy-1 with reduced glycoform distribution by MALDI-TOF and ESI FT/ICR MS of AspN digested glycoprotein. 467-468. Paper presented at Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States.