Characterizing recombinant chicken Thy-1 with reduced glycoform distribution by MALDI-TOF and ESI FT/ICR MS of AspN digested glycoprotein

Promod Mehndiratta; Silvia M. Pulido; Wendy J. Walton; Mark R. Emmett; Joan Hare; Alan G. Marshall; M. Timothy Logan

Characterizing recombinant chicken Thy-1 with reduced glycoform distribution by MALDI-TOF and ESI FT/ICR MS of AspN digested glycoprotein

Promod Mehndiratta
, Silvia M. Pulido
, Wendy J. Walton
, Mark R. Emmett
, Joan Hare
, Alan G. Marshall
, M. Timothy Logan

Research output: Contribution to conference › Paper › peer-review

Abstract

A method was developed to enhance the carbohydrate homogeneity of recombinant protein. The secreted protein was purified using a combination of ultrafiltration, lectin affinity chromatography, ion exchange chromatography and reverse-phase HPLC. The disulphide linkages and the glycoform distribution on all three glycosylation sites were characterized by combining N-terminal sequencing with matrix-assisted laser desorption ionization time of flight (MALDI-TOF) and ESI FT-ICR MS. The techniques confirmed the expected disulphide linkage pattern.

Original language	English (US)
Pages	467-468
Number of pages	2
State	Published - 2002
Externally published	Yes
Event	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States Duration: Jun 2 2002 → Jun 6 2002

Other

Other	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
Country/Territory	United States
City	Orlando, FL
Period	6/2/02 → 6/6/02

ASJC Scopus subject areas

Spectroscopy

Cite this

Mehndiratta, P., Pulido, S. M., Walton, W. J., Emmett, M. R., Hare, J., Marshall, A. G., & Logan, M. T. (2002). Characterizing recombinant chicken Thy-1 with reduced glycoform distribution by MALDI-TOF and ESI FT/ICR MS of AspN digested glycoprotein. 467-468. Paper presented at Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States.

Characterizing recombinant chicken Thy-1 with reduced glycoform distribution by MALDI-TOF and ESI FT/ICR MS of AspN digested glycoprotein. / Mehndiratta, Promod; Pulido, Silvia M.; Walton, Wendy J. et al.
2002. 467-468 Paper presented at Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States.

Research output: Contribution to conference › Paper › peer-review

Mehndiratta, P, Pulido, SM, Walton, WJ, Emmett, MR, Hare, J, Marshall, AG & Logan, MT 2002, 'Characterizing recombinant chicken Thy-1 with reduced glycoform distribution by MALDI-TOF and ESI FT/ICR MS of AspN digested glycoprotein', Paper presented at Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States, 6/2/02 - 6/6/02 pp. 467-468.

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title = "Characterizing recombinant chicken Thy-1 with reduced glycoform distribution by MALDI-TOF and ESI FT/ICR MS of AspN digested glycoprotein",

abstract = "A method was developed to enhance the carbohydrate homogeneity of recombinant protein. The secreted protein was purified using a combination of ultrafiltration, lectin affinity chromatography, ion exchange chromatography and reverse-phase HPLC. The disulphide linkages and the glycoform distribution on all three glycosylation sites were characterized by combining N-terminal sequencing with matrix-assisted laser desorption ionization time of flight (MALDI-TOF) and ESI FT-ICR MS. The techniques confirmed the expected disulphide linkage pattern.",

author = "Promod Mehndiratta and Pulido, \{Silvia M.\} and Walton, \{Wendy J.\} and Emmett, \{Mark R.\} and Joan Hare and Marshall, \{Alan G.\} and Logan, \{M. Timothy\}",

year = "2002",

language = "English (US)",

pages = "467--468",

note = "Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics ; Conference date: 02-06-2002 Through 06-06-2002",

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AU - Mehndiratta, Promod

AU - Pulido, Silvia M.

AU - Walton, Wendy J.

AU - Emmett, Mark R.

AU - Hare, Joan

AU - Marshall, Alan G.

AU - Logan, M. Timothy

PY - 2002

Y1 - 2002

N2 - A method was developed to enhance the carbohydrate homogeneity of recombinant protein. The secreted protein was purified using a combination of ultrafiltration, lectin affinity chromatography, ion exchange chromatography and reverse-phase HPLC. The disulphide linkages and the glycoform distribution on all three glycosylation sites were characterized by combining N-terminal sequencing with matrix-assisted laser desorption ionization time of flight (MALDI-TOF) and ESI FT-ICR MS. The techniques confirmed the expected disulphide linkage pattern.

AB - A method was developed to enhance the carbohydrate homogeneity of recombinant protein. The secreted protein was purified using a combination of ultrafiltration, lectin affinity chromatography, ion exchange chromatography and reverse-phase HPLC. The disulphide linkages and the glycoform distribution on all three glycosylation sites were characterized by combining N-terminal sequencing with matrix-assisted laser desorption ionization time of flight (MALDI-TOF) and ESI FT-ICR MS. The techniques confirmed the expected disulphide linkage pattern.

UR - https://www.scopus.com/pages/publications/2442654460

UR - https://www.scopus.com/pages/publications/2442654460#tab=citedBy

M3 - Paper

AN - SCOPUS:2442654460

SP - 467

EP - 468

T2 - Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics

Y2 - 2 June 2002 through 6 June 2002

ER -

Characterizing recombinant chicken Thy-1 with reduced glycoform distribution by MALDI-TOF and ESI FT/ICR MS of AspN digested glycoprotein

Abstract

Other

ASJC Scopus subject areas

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