Characterizing the propionibacterium load in revision shoulder arthroplasty a study of 137 culture-positive cases

Zahab S. Ahsan, Jeremy Somerson, Frederick A. Matsen

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Background: Propionibacterium is commonly recovered from explants or surrounding tissues in revision shoulder arthroplasty. Rather than attempting to differentiate a true infection from a false-positive result on the basis of the number of positive cultures, we characterized the amount of these bacteria in each specimen and shoulder. Methods: The study included 137 revision shoulder arthroplasties from which a minimum of 4 specimens had been submitted for culture and at least 1 was positive for Propionibacterium. Standard microbiology procedures were used to assign a semiquantitative value (0.1, 1, 2, 3, or 4), called the Specimen Propi Value, to the amount of growth in each specimen. The sum of the Specimen Propi Values for each shoulder was defined as the Shoulder Propi Score, which was then divided by the total number of specimens to calculate the Average Shoulder Propi Score. Results: The number and percentage of positive specimen-specific cultures (of material obtained from the stem explant, head explant, glenoid explant, humeral membrane, collar membrane, other soft tissue, fluid, or other) per shoulder ranged from 1 to 6 and 14% to 100%. A high percentage of specimens (mean, 43%; median, 50%) from the culture-positive shoulders showed no growth. Only 32.6% of the fluid cultures were positive in comparison with 66.5% of the soft-tissue cultures and 55.6% of the cultures of explant specimens. The average Specimen Propi Value (and standard deviation) for fluid specimens (0.35 ± 0.89) was significantly lower than those for the soft-tissue (0.92 ± 1.50) and explant (0.66 ± 0.90) specimens (p > 0.001). The Shoulder Propi Score was significantly higher in men (3.56 ± 3.74) than in women (1.22± 3.11) (p > 0.001). Similarly, men had a significantly higher Average Shoulder Propi Score (0.53 ± 0.51) than women (0.19 ± 0.43) (p > 0.001). Conclusions: This investigation suggests that Propionibacterium is unevenly distributed within culture-positive revised shoulders. As a result, the specimen number and source (explant, soft tissue, or fluid) have major influences on the culture results for a revised shoulder arthroplasty. We found no evidence that suggested useful threshold values for defining a true infection. Level of Evidence: Diagnostic Level III. See Instructions for Authors for a complete description of levels of evidence.

Original languageEnglish (US)
Pages (from-to)150-154
Number of pages5
JournalJournal of Bone and Joint Surgery - American Volume
Volume99
Issue number2
DOIs
StatePublished - 2017

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Propionibacterium
Arthroplasty
Membranes
Growth
Microbiology
Infection

ASJC Scopus subject areas

  • Surgery
  • Medicine(all)
  • Orthopedics and Sports Medicine

Cite this

Characterizing the propionibacterium load in revision shoulder arthroplasty a study of 137 culture-positive cases. / Ahsan, Zahab S.; Somerson, Jeremy; Matsen, Frederick A.

In: Journal of Bone and Joint Surgery - American Volume, Vol. 99, No. 2, 2017, p. 150-154.

Research output: Contribution to journalArticle

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abstract = "Background: Propionibacterium is commonly recovered from explants or surrounding tissues in revision shoulder arthroplasty. Rather than attempting to differentiate a true infection from a false-positive result on the basis of the number of positive cultures, we characterized the amount of these bacteria in each specimen and shoulder. Methods: The study included 137 revision shoulder arthroplasties from which a minimum of 4 specimens had been submitted for culture and at least 1 was positive for Propionibacterium. Standard microbiology procedures were used to assign a semiquantitative value (0.1, 1, 2, 3, or 4), called the Specimen Propi Value, to the amount of growth in each specimen. The sum of the Specimen Propi Values for each shoulder was defined as the Shoulder Propi Score, which was then divided by the total number of specimens to calculate the Average Shoulder Propi Score. Results: The number and percentage of positive specimen-specific cultures (of material obtained from the stem explant, head explant, glenoid explant, humeral membrane, collar membrane, other soft tissue, fluid, or other) per shoulder ranged from 1 to 6 and 14{\%} to 100{\%}. A high percentage of specimens (mean, 43{\%}; median, 50{\%}) from the culture-positive shoulders showed no growth. Only 32.6{\%} of the fluid cultures were positive in comparison with 66.5{\%} of the soft-tissue cultures and 55.6{\%} of the cultures of explant specimens. The average Specimen Propi Value (and standard deviation) for fluid specimens (0.35 ± 0.89) was significantly lower than those for the soft-tissue (0.92 ± 1.50) and explant (0.66 ± 0.90) specimens (p > 0.001). The Shoulder Propi Score was significantly higher in men (3.56 ± 3.74) than in women (1.22± 3.11) (p > 0.001). Similarly, men had a significantly higher Average Shoulder Propi Score (0.53 ± 0.51) than women (0.19 ± 0.43) (p > 0.001). Conclusions: This investigation suggests that Propionibacterium is unevenly distributed within culture-positive revised shoulders. As a result, the specimen number and source (explant, soft tissue, or fluid) have major influences on the culture results for a revised shoulder arthroplasty. We found no evidence that suggested useful threshold values for defining a true infection. Level of Evidence: Diagnostic Level III. See Instructions for Authors for a complete description of levels of evidence.",
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