Chemical identification and evaluation of pheromones from the axial breeding glands of African clawed frog hymenochirus using electrospray mass spectrometry and associated techniques

Yu Wang, Carson Lawall, Kangling Zhang, Chris Cox, O. David Sparkman, Eric O. Thomas, Patrick R. Jones

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Phermones from the axial breeding glands of African clawed frog hymenochirus was chemically identified and evaluated using electrospray mass spectrometry. Ten selected male frogs were injected with Gonadotropin Releasing hormone (GnRH) twelve hours prior to the experiment. The male and female gland secretions were analyzed by a Schimadzu high performance liquid chromatography (HPLC) using a water-acetonitrile-formic acid gradient system. It was found that the female frogs were more attracted to water housing males than to water housing females and to water containing homogenized breeding glands than to water housing breeding gland-ablated males.

Original languageEnglish (US)
Title of host publicationProceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics
Pages359-360
Number of pages2
StatePublished - 2002
Externally publishedYes
EventProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States
Duration: Jun 2 2002Jun 6 2002

Other

OtherProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
CountryUnited States
CityOrlando, FL
Period6/2/026/6/02

Fingerprint

Pheromones
Mass spectrometry
Water
formic acid
High performance liquid chromatography
Gonadotropin-Releasing Hormone
Experiments

ASJC Scopus subject areas

  • Spectroscopy

Cite this

Wang, Y., Lawall, C., Zhang, K., Cox, C., Sparkman, O. D., Thomas, E. O., & Jones, P. R. (2002). Chemical identification and evaluation of pheromones from the axial breeding glands of African clawed frog hymenochirus using electrospray mass spectrometry and associated techniques. In Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics (pp. 359-360)

Chemical identification and evaluation of pheromones from the axial breeding glands of African clawed frog hymenochirus using electrospray mass spectrometry and associated techniques. / Wang, Yu; Lawall, Carson; Zhang, Kangling; Cox, Chris; Sparkman, O. David; Thomas, Eric O.; Jones, Patrick R.

Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. 2002. p. 359-360.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Wang, Y, Lawall, C, Zhang, K, Cox, C, Sparkman, OD, Thomas, EO & Jones, PR 2002, Chemical identification and evaluation of pheromones from the axial breeding glands of African clawed frog hymenochirus using electrospray mass spectrometry and associated techniques. in Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. pp. 359-360, Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States, 6/2/02.
Wang Y, Lawall C, Zhang K, Cox C, Sparkman OD, Thomas EO et al. Chemical identification and evaluation of pheromones from the axial breeding glands of African clawed frog hymenochirus using electrospray mass spectrometry and associated techniques. In Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. 2002. p. 359-360
Wang, Yu ; Lawall, Carson ; Zhang, Kangling ; Cox, Chris ; Sparkman, O. David ; Thomas, Eric O. ; Jones, Patrick R. / Chemical identification and evaluation of pheromones from the axial breeding glands of African clawed frog hymenochirus using electrospray mass spectrometry and associated techniques. Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. 2002. pp. 359-360
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