Chemiluminescence-based electrophoretic mobility shift assay of RNA-protein interactions: Application to binding of viral capsid proteins to RNA

Jonghoon Kang, Myung Soog Lee, Stanley J. Watowich, David G. Gorenstein

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

A chemiluminescence electrophoretic mobility shift assay was introduced for the study of RNA-protein interactions that include association of genomic RNA and viral capsid proteins. Binding of the capsid protein of Venezuelan equine encephalitis virus (VEEV) to several types of RNA was used as a model system to test the application of the method. The effects of RNA secondary structures and the significance of electrostatic interaction on binding were identified. This method may have wide application to the study of RNA-protein interactions.

Original languageEnglish (US)
Pages (from-to)155-159
Number of pages5
JournalJournal of Virological Methods
Volume131
Issue number2
DOIs
StatePublished - Feb 1 2006

Keywords

  • Capsid
  • Chemiluminescence
  • EMSA
  • RNA
  • Venezuelan equine encephalitis virus

ASJC Scopus subject areas

  • Virology

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