Chemokine-dependent mechanisms of leukocyte trafficking across a model of the blood-brain barrier

Eliseo Eugenin, Joan W. Berman

Research output: Contribution to journalArticle

97 Citations (Scopus)

Abstract

Leukocyte transmigration across the blood-brain barrier (BBB) is a multistep process that can be mediated by chemokines. These low-molecular-weight chemoattractant proteins are secreted by cells within the central nervous system (CNS) in response to injury or on activation. Leukocytes transmigrate toward this chemokine gradient, crossing the BBB and gaining access to the CNS parenchyma. Depending on the chemokine, the nature of the insult, and the type of cell that transmigrates, the BBB integrity may be disrupted, leading to its increased permeability. Both the inflammation resulting from leukocyte transmigration and BBB perturbations contribute to CNS pathology. The mechanisms that mediate leukocyte transmigration and BBB disruption, as well as tissue culture models that are used to study leukocyte trafficking, are the focus of this review.

Original languageEnglish (US)
Pages (from-to)351-361
Number of pages11
JournalMethods
Volume29
Issue number4
DOIs
StatePublished - Apr 1 2003
Externally publishedYes

Fingerprint

Blood-Brain Barrier
Chemokines
Leukocytes
Neurology
Central Nervous System
Tissue culture
Chemotactic Factors
Pathology
Permeability
Molecular Weight
Chemical activation
Molecular weight
Inflammation
Wounds and Injuries
Proteins

Keywords

  • Astrocyte
  • BBB
  • Chemotaxis
  • Endothelial
  • Leukocyte
  • PBMC
  • Transmigration
  • Transwell

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Chemokine-dependent mechanisms of leukocyte trafficking across a model of the blood-brain barrier. / Eugenin, Eliseo; Berman, Joan W.

In: Methods, Vol. 29, No. 4, 01.04.2003, p. 351-361.

Research output: Contribution to journalArticle

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