Chikungunya outbreaks in India: A prospective study comparing neutralization and sequelae during two outbreaks in 2010 and 2016

Jaspreet Jain, Navjot Kaur, Sherry L. Haller, Ankit Kumar, Shannan L. Rossi, Vimal Narayanan, Dilip Kumar, Rajni Gaind, Scott C. Weaver, Albert J. Auguste, Sujatha Sunil

Research output: Contribution to journalReview article


Chikungunya fever (CHIKF) is a major public health concern and is caused by chikungunya virus (CHIKV). In 2005, the virus was reintroduced into India, resulting in massive outbreaks in several parts of the country. During 2010 and 2016 outbreaks, we recruited 588 patients from a tertiary care hospital in New Delhi, India, during the acute phase of CHIKF; collected their blood and clinical data; and determined their arthralgic status 12 weeks post-onset of fever. We evaluated IgM/IgG CHIKV-binding antibodies and their neutralizing capacity, sequenced complete genomes of 21 CHIKV strains, and correlated mutations with patient sequelae status. We also performed infections in murine models using representative strains from each outbreak to evaluate differences in pathogenesis. Our screening and analysis revealed that patients of the 2016 outbreak developed earlier IgM and neutralizing antibody responses that were negatively correlated with sequelae, compared with 2010 patients. Mutations that correlated with human disease progression were also correlated with enhanced murine virulence and pathogenesis. Overall, our study suggests that the development of early neutralizing antibodies and sequence variation in clinical isolates are predictors of human sequelae.

Original languageEnglish (US)
Pages (from-to)857-868
Number of pages12
JournalAmerican Journal of Tropical Medicine and Hygiene
Issue number4
StatePublished - Jan 1 2020


ASJC Scopus subject areas

  • Parasitology
  • Virology
  • Infectious Diseases

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