Chimeric chikungunya viruses are nonpathogenic in highly sensitive mouse models but efficiently induce a protective immune response

Eryu Wang, Dal Young Kim, Scott Weaver, Ilya Frolov

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Chikungunya virus (CHIKV) is an important pathogen causing outbreaks of highly debilitating and often chronic, arthralgic human disease. We have designed chimeric alphaviruses encoding CHIKV-specific structural proteins but no structural or nonstructural proteins capable of interfering with development of cellular antiviral response. These chimeras demonstrate a highly attenuated phenotype in both immunocompetent and immunocompromised (A129) mice. However, after a single vaccination, they induced protective immune response against subsequent CHIKV challenge, characterized by high titers of neutralizing antibodies. The rational design of alphavirus genomes provides a strong basis for the development of new recombinant alphaviruses with irreversible, highly attenuated, cell type-restricted phenotypes.

Original languageEnglish (US)
Pages (from-to)9249-9252
Number of pages4
JournalJournal of Virology
Volume85
Issue number17
DOIs
StatePublished - Sep 2011

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Chikungunya virus
Alphavirus
animal models
immune response
Viral Structural Proteins
Phenotype
phenotype
chimerism
structural proteins
Neutralizing Antibodies
neutralizing antibodies
human diseases
Antiviral Agents
Disease Outbreaks
Vaccination
vaccination
Genome
genome
pathogens
mice

ASJC Scopus subject areas

  • Immunology
  • Virology

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Chimeric chikungunya viruses are nonpathogenic in highly sensitive mouse models but efficiently induce a protective immune response. / Wang, Eryu; Kim, Dal Young; Weaver, Scott; Frolov, Ilya.

In: Journal of Virology, Vol. 85, No. 17, 09.2011, p. 9249-9252.

Research output: Contribution to journalArticle

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