Chimeric Sindbis/eastern equine encephalitis vaccine candidates are highly attenuated and immunogenic in mice

Eryu Wang, Olga Petrakova, A. Paige Adams, Patricia V. Aguilar, Wenli Kang, Slobodan Paessler, Sara M. Volk, Ilya Frolov, Scott C. Weaver

Research output: Contribution to journalArticlepeer-review

60 Scopus citations

Abstract

We developed chimeric Sindbis (SINV)/eastern equine encephalitis (EEEV) viruses and investigated their potential for use as live virus vaccines against EEEV. One vaccine candidate contained structural protein genes from a typical North American EEEV strain, while the other had structural proteins from a naturally attenuated Brazilian isolate. Both chimeric viruses replicated efficiently in mammalian and mosquito cell cultures and were highly attenuated in mice. Vaccinated mice did not develop detectable disease or viremia, but developed high titers of neutralizing antibodies. Upon challenge with EEEV, mice vaccinated with >104 PFU of the chimeric viruses were completely protected from disease. These findings support the potential use of these SIN/EEEV chimeras as safe and effective vaccines.

Original languageEnglish (US)
Pages (from-to)7573-7581
Number of pages9
JournalVaccine
Volume25
Issue number43
DOIs
StatePublished - Oct 23 2007

Keywords

  • Alphavirus
  • Encephalitis
  • Vaccine

ASJC Scopus subject areas

  • Molecular Medicine
  • Immunology and Microbiology(all)
  • veterinary(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

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