Chimeric Sindbis/eastern equine encephalitis vaccine candidates are highly attenuated and immunogenic in mice

Eryu Wang; Olga Petrakova; A. Paige Adams; Patricia V. Aguilar; Wenli Kang; Slobodan Paessler; Sara M. Volk; Ilya Frolov; Scott C. Weaver

doi:10.1016/j.vaccine.2007.07.061

Chimeric Sindbis/eastern equine encephalitis vaccine candidates are highly attenuated and immunogenic in mice

Eryu Wang, Olga Petrakova, A. Paige Adams, Patricia V. Aguilar, Wenli Kang, Slobodan Paessler, Sara M. Volk, Ilya Frolov, Scott C. Weaver

Research output: Contribution to journal › Article › peer-review

73 Scopus citations

Abstract

We developed chimeric Sindbis (SINV)/eastern equine encephalitis (EEEV) viruses and investigated their potential for use as live virus vaccines against EEEV. One vaccine candidate contained structural protein genes from a typical North American EEEV strain, while the other had structural proteins from a naturally attenuated Brazilian isolate. Both chimeric viruses replicated efficiently in mammalian and mosquito cell cultures and were highly attenuated in mice. Vaccinated mice did not develop detectable disease or viremia, but developed high titers of neutralizing antibodies. Upon challenge with EEEV, mice vaccinated with >10⁴ PFU of the chimeric viruses were completely protected from disease. These findings support the potential use of these SIN/EEEV chimeras as safe and effective vaccines.

Original language	English (US)
Pages (from-to)	7573-7581
Number of pages	9
Journal	Vaccine
Volume	25
Issue number	43
DOIs	https://doi.org/10.1016/j.vaccine.2007.07.061
State	Published - Oct 23 2007

Keywords

Alphavirus
Encephalitis
Vaccine

ASJC Scopus subject areas

Molecular Medicine
General Immunology and Microbiology
General Veterinary
Public Health, Environmental and Occupational Health
Infectious Diseases

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10.1016/j.vaccine.2007.07.061

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title = "Chimeric Sindbis/eastern equine encephalitis vaccine candidates are highly attenuated and immunogenic in mice",

abstract = "We developed chimeric Sindbis (SINV)/eastern equine encephalitis (EEEV) viruses and investigated their potential for use as live virus vaccines against EEEV. One vaccine candidate contained structural protein genes from a typical North American EEEV strain, while the other had structural proteins from a naturally attenuated Brazilian isolate. Both chimeric viruses replicated efficiently in mammalian and mosquito cell cultures and were highly attenuated in mice. Vaccinated mice did not develop detectable disease or viremia, but developed high titers of neutralizing antibodies. Upon challenge with EEEV, mice vaccinated with >104 PFU of the chimeric viruses were completely protected from disease. These findings support the potential use of these SIN/EEEV chimeras as safe and effective vaccines.",

keywords = "Alphavirus, Encephalitis, Vaccine",

author = "Eryu Wang and Olga Petrakova and Adams, {A. Paige} and Aguilar, {Patricia V.} and Wenli Kang and Slobodan Paessler and Volk, {Sara M.} and Ilya Frolov and Weaver, {Scott C.}",

note = "Funding Information: We thank Anna Grund and Melinda Kelley for assistance with the implantation of telemetry chips and transferring temperature data from mice, and Eric Fokam for help with serological assays. Nancy Davis and Robert Johnston kindly provided the V3526 cDNA clone. This work was supported by a grant from NIAID through the Western Regional Center of Excellence for Biodefense and Emerging Infectious Diseases Research, NIH grant number U54 AI057156. PVA and APA were supported by the James W. McLaughlin fellowship fund. SP was supported by NIH K08 grant no. AI059491.",

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doi = "10.1016/j.vaccine.2007.07.061",

language = "English (US)",

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AU - Wang, Eryu

AU - Petrakova, Olga

AU - Adams, A. Paige

AU - Aguilar, Patricia V.

AU - Kang, Wenli

AU - Paessler, Slobodan

AU - Volk, Sara M.

AU - Frolov, Ilya

AU - Weaver, Scott C.

N1 - Funding Information: We thank Anna Grund and Melinda Kelley for assistance with the implantation of telemetry chips and transferring temperature data from mice, and Eric Fokam for help with serological assays. Nancy Davis and Robert Johnston kindly provided the V3526 cDNA clone. This work was supported by a grant from NIAID through the Western Regional Center of Excellence for Biodefense and Emerging Infectious Diseases Research, NIH grant number U54 AI057156. PVA and APA were supported by the James W. McLaughlin fellowship fund. SP was supported by NIH K08 grant no. AI059491.

PY - 2007/10/23

Y1 - 2007/10/23

N2 - We developed chimeric Sindbis (SINV)/eastern equine encephalitis (EEEV) viruses and investigated their potential for use as live virus vaccines against EEEV. One vaccine candidate contained structural protein genes from a typical North American EEEV strain, while the other had structural proteins from a naturally attenuated Brazilian isolate. Both chimeric viruses replicated efficiently in mammalian and mosquito cell cultures and were highly attenuated in mice. Vaccinated mice did not develop detectable disease or viremia, but developed high titers of neutralizing antibodies. Upon challenge with EEEV, mice vaccinated with >104 PFU of the chimeric viruses were completely protected from disease. These findings support the potential use of these SIN/EEEV chimeras as safe and effective vaccines.

AB - We developed chimeric Sindbis (SINV)/eastern equine encephalitis (EEEV) viruses and investigated their potential for use as live virus vaccines against EEEV. One vaccine candidate contained structural protein genes from a typical North American EEEV strain, while the other had structural proteins from a naturally attenuated Brazilian isolate. Both chimeric viruses replicated efficiently in mammalian and mosquito cell cultures and were highly attenuated in mice. Vaccinated mice did not develop detectable disease or viremia, but developed high titers of neutralizing antibodies. Upon challenge with EEEV, mice vaccinated with >104 PFU of the chimeric viruses were completely protected from disease. These findings support the potential use of these SIN/EEEV chimeras as safe and effective vaccines.

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KW - Vaccine

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Chimeric Sindbis/eastern equine encephalitis vaccine candidates are highly attenuated and immunogenic in mice

Abstract

Keywords

ASJC Scopus subject areas

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