Chimeric Sindbis/eastern equine encephalitis vaccine candidates are highly attenuated and immunogenic in mice

Eryu Wang, Olga Petrakova, A. Paige Adams, Patricia Aguilar, Wenli Kang, Slobodan Paessler, Sara M. Volk, Ilya Frolov, Scott Weaver

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

We developed chimeric Sindbis (SINV)/eastern equine encephalitis (EEEV) viruses and investigated their potential for use as live virus vaccines against EEEV. One vaccine candidate contained structural protein genes from a typical North American EEEV strain, while the other had structural proteins from a naturally attenuated Brazilian isolate. Both chimeric viruses replicated efficiently in mammalian and mosquito cell cultures and were highly attenuated in mice. Vaccinated mice did not develop detectable disease or viremia, but developed high titers of neutralizing antibodies. Upon challenge with EEEV, mice vaccinated with >104 PFU of the chimeric viruses were completely protected from disease. These findings support the potential use of these SIN/EEEV chimeras as safe and effective vaccines.

Original languageEnglish (US)
Pages (from-to)7573-7581
Number of pages9
JournalVaccine
Volume25
Issue number43
DOIs
StatePublished - Oct 23 2007

Fingerprint

Eastern Equine Encephalomyelitis
encephalitis
Vaccines
structural proteins
vaccines
Viruses
horses
viruses
mice
Eastern equine encephalitis virus
chimerism
Viremia
viremia
Neutralizing Antibodies
Culicidae
neutralizing antibodies
Proteins
cell culture
Cell Culture Techniques
genes

Keywords

  • Alphavirus
  • Encephalitis
  • Vaccine

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Virology
  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • veterinary(all)

Cite this

Chimeric Sindbis/eastern equine encephalitis vaccine candidates are highly attenuated and immunogenic in mice. / Wang, Eryu; Petrakova, Olga; Adams, A. Paige; Aguilar, Patricia; Kang, Wenli; Paessler, Slobodan; Volk, Sara M.; Frolov, Ilya; Weaver, Scott.

In: Vaccine, Vol. 25, No. 43, 23.10.2007, p. 7573-7581.

Research output: Contribution to journalArticle

Wang, Eryu ; Petrakova, Olga ; Adams, A. Paige ; Aguilar, Patricia ; Kang, Wenli ; Paessler, Slobodan ; Volk, Sara M. ; Frolov, Ilya ; Weaver, Scott. / Chimeric Sindbis/eastern equine encephalitis vaccine candidates are highly attenuated and immunogenic in mice. In: Vaccine. 2007 ; Vol. 25, No. 43. pp. 7573-7581.
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