Chimpanzee adenovirus vaccine protects against Zaire Ebola virus

Gary P. Kobinger, Heinz Feldmann, Yan Zhi, Gregory Schumer, Guangping Gao, Friederike Feldmann, Steven Jones, James M. Wilson

Research output: Contribution to journalArticlepeer-review

116 Scopus citations

Abstract

This study evaluated the use of a chimpanzee-based adenovirus vaccine in mouse and Guinea pigs models of Zaire Ebola virus (ZEBOV) infection. Vaccine vector expressing the envelope glycoprotein of ZEBOV was created from the molecular clone of chimpanzee adenovirus pan7 (AdC7). AdC7 vaccine stimulated robust T and B cell responses to ZEBOV in naïve mice inducing complete protection to an otherwise lethal challenge of ZEBOV. Complete protection to Zaire Ebola virus was also observed in Guinea pigs vaccinated with a relatively low dose of AdC7 (5 × 109/kg). Pre-existing immunity to AdHu5 was generated in mice following pre-exposure to AdHu5 or administration of pooled human immune globulin. Pre-existing immunity to human adenoviruses severely compromised the efficacy of the human AdHu5 vaccine but not the chimpanzee AdC7 vaccine. These results validate further development of Chimpanzee-based vaccine and highlight the impact of pre-existing immunity to the vaccine carrier.

Original languageEnglish (US)
Pages (from-to)394-401
Number of pages8
JournalVirology
Volume346
Issue number2
DOIs
StatePublished - Mar 15 2006
Externally publishedYes

Keywords

  • Adenovirus
  • Chimpanzee
  • Ebola
  • Guinea pig
  • Mice
  • Preexisting immunity
  • Vaccine

ASJC Scopus subject areas

  • Virology

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