Chloroquine is a potent inhibitor of SARS coronavirus infection and spread

Martin J. Vincent, Eric Bergeron, Suzanne Benjannet, Bobbie R. Erickson, Pierre E. Rollin, Thomas Ksiazek, Nabil G. Seidah, Stuart T. Nichol

Research output: Contribution to journalArticle

75 Citations (Scopus)

Abstract

Background: Severe acute respiratory syndrome (SARS) is caused by a newly discovered coronavirus (SARS-CoV). No effective prophylactic or post-exposure therapy is currently available. Results: We report, however, that chloroquine has strong antiviral effects on SARS-CoV infection of primate cells. These inhibitory effects are observed when the cells are treated with the drug either before or after exposure to the virus, suggesting both prophylactic and therapeutic advantage. In addition to the well-known functions of chloroquine such as elevations of endosomal pH, the drug appears to interfere with terminal glycosylation of the cellular receptor, angiotensin-converting enzyme 2. This may negatively influence the virus-receptor binding and abrogate the infection, with further ramifications by the elevation of vesicular pH, resulting in the inhibition of infection and spread of SARS CoV at clinically admissible concentrations. Conclusion: Chloroquine is effective in preventing the spread of SARS CoV in cell culture. Favorable inhibition of virus spread was observed when the cells were either treated with chloroquine prior to or after SARS CoV infection. In addition, the indirect immunofluorescence assay described herein represents a simple and rapid method for screening SARS-CoV antiviral compounds.

Original languageEnglish (US)
JournalVirology Journal
Volume2
DOIs
StatePublished - Aug 22 2005
Externally publishedYes

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Coronavirus Infections
Severe Acute Respiratory Syndrome
Chloroquine
Infection
Antiviral Agents
Implosive Therapy
Viruses
Virus Receptors
Virus Attachment
Coronavirus
Indirect Fluorescent Antibody Technique
Glycosylation
Pharmaceutical Preparations
Primates
Cell Culture Techniques

ASJC Scopus subject areas

  • Virology
  • Genetics(clinical)
  • Infectious Diseases

Cite this

Vincent, M. J., Bergeron, E., Benjannet, S., Erickson, B. R., Rollin, P. E., Ksiazek, T., ... Nichol, S. T. (2005). Chloroquine is a potent inhibitor of SARS coronavirus infection and spread. Virology Journal, 2. https://doi.org/10.1186/1743-422X-2-69

Chloroquine is a potent inhibitor of SARS coronavirus infection and spread. / Vincent, Martin J.; Bergeron, Eric; Benjannet, Suzanne; Erickson, Bobbie R.; Rollin, Pierre E.; Ksiazek, Thomas; Seidah, Nabil G.; Nichol, Stuart T.

In: Virology Journal, Vol. 2, 22.08.2005.

Research output: Contribution to journalArticle

Vincent, MJ, Bergeron, E, Benjannet, S, Erickson, BR, Rollin, PE, Ksiazek, T, Seidah, NG & Nichol, ST 2005, 'Chloroquine is a potent inhibitor of SARS coronavirus infection and spread', Virology Journal, vol. 2. https://doi.org/10.1186/1743-422X-2-69
Vincent, Martin J. ; Bergeron, Eric ; Benjannet, Suzanne ; Erickson, Bobbie R. ; Rollin, Pierre E. ; Ksiazek, Thomas ; Seidah, Nabil G. ; Nichol, Stuart T. / Chloroquine is a potent inhibitor of SARS coronavirus infection and spread. In: Virology Journal. 2005 ; Vol. 2.
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