CHO-Omics Review

The Impact of Current and Emerging Technologies on Chinese Hamster Ovary Based Bioproduction

Gino Stolfa, Matthew T. Smonskey, Ryan Boniface, Anna Barbara Hachmann, Paul Gulde, Atul D. Joshi, Anson P. Pierce, Scott J. Jacobia, Andrew Campbell

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

CHO cells are the most prevalent platform for modern bio-therapeutic production. Currently, there are several CHO cell lines used in bioproduction with distinct characteristics and unique genotypes and phenotypes. These differences limit advances in productivity and quality that can be achieved by the most common approaches to bioprocess optimization and cell line engineering. Incorporating omics-based approaches into current bioproduction processes will complement traditional methodologies to maximize gains from CHO engineering and bioprocess improvements. In order to highlight the utility of omics technologies in CHO bioproduction, the authors discuss current applications as well as limitations of genomics, transcriptomics, proteomics, metabolomics, lipidomics, fluxomics, glycomics, and multi-omics approaches and the potential they hold for the future of bioproduction. Multiple omics approaches are currently being used to improve CHO bioprocesses; however, the application of these technologies is still limited. As more CHO-omic datasets become available and integrated into systems models, the authors expect significant gains in product yield and quality. While individual omics technologies provide incremental improvements in bioproduction, the authors will likely see the most significant gains by applying multi-omics and systems biology approaches to individual CHO cell lines.

Original languageEnglish (US)
JournalBiotechnology Journal
DOIs
StateAccepted/In press - Jan 1 2017
Externally publishedYes

Fingerprint

CHO Cells
Cricetulus
Ovary
Technology
Cell Line
Glycomics
Cell Engineering
Metabolomics
Systems Biology
Genomics
Proteomics
Genotype
Phenotype
Therapeutics

Keywords

  • Bioproduction
  • Cell line engineering
  • CHO
  • Media development
  • Omics

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Molecular Medicine

Cite this

Stolfa, G., Smonskey, M. T., Boniface, R., Hachmann, A. B., Gulde, P., Joshi, A. D., ... Campbell, A. (Accepted/In press). CHO-Omics Review: The Impact of Current and Emerging Technologies on Chinese Hamster Ovary Based Bioproduction. Biotechnology Journal. https://doi.org/10.1002/biot.201700227

CHO-Omics Review : The Impact of Current and Emerging Technologies on Chinese Hamster Ovary Based Bioproduction. / Stolfa, Gino; Smonskey, Matthew T.; Boniface, Ryan; Hachmann, Anna Barbara; Gulde, Paul; Joshi, Atul D.; Pierce, Anson P.; Jacobia, Scott J.; Campbell, Andrew.

In: Biotechnology Journal, 01.01.2017.

Research output: Contribution to journalArticle

Stolfa, G, Smonskey, MT, Boniface, R, Hachmann, AB, Gulde, P, Joshi, AD, Pierce, AP, Jacobia, SJ & Campbell, A 2017, 'CHO-Omics Review: The Impact of Current and Emerging Technologies on Chinese Hamster Ovary Based Bioproduction', Biotechnology Journal. https://doi.org/10.1002/biot.201700227
Stolfa, Gino ; Smonskey, Matthew T. ; Boniface, Ryan ; Hachmann, Anna Barbara ; Gulde, Paul ; Joshi, Atul D. ; Pierce, Anson P. ; Jacobia, Scott J. ; Campbell, Andrew. / CHO-Omics Review : The Impact of Current and Emerging Technologies on Chinese Hamster Ovary Based Bioproduction. In: Biotechnology Journal. 2017.
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