Cholecystokinin secretagogue-induced gastroprotection: Role of nitric oxide and blood flow

Sonlee D. West, Kenneth S. Helmer, Lily K. Chang, Yan Cui, George H. Greeley, David W. Mercer

    Research output: Contribution to journalArticle

    31 Scopus citations

    Abstract

    This study was done to examine the role of CCK in gastric mucosal defense and to assess the gastroprotective roles of nitric oxide and blood flow. In rats, the CCK secretagogues oleate and soybean trypsin inhibitor augmented gastric mucosal blood flow and prevented gastric injury from luminal irritants. Type A CCK receptor blockade negated CCK secretagogue-induced gastroprotection and exacerbated gastric injury from bile and ethanol but did not block adaptive cytoprotection. CCK secretagogue-induced gastroprotection and hyperemia were negated by nonselective nitric oxide synthase (NOS) inhibition (nG-nitro-L-arginine methyl ester) but not by selective inducible NOS inhibition (aminoguanidine). Gastric mucosal calcium-dependent NOS activity, but not calcium-independent NOS activity, was increased following CCK and CCK secretagogues. The release of endogenous CCK plays a role in the intrinsic gastric mucosal defense system against injury from luminal irritants. The protective mechanism appears to involve increased production of nitric oxide from primarily the constitutive isoforms of NOS and a resultant increase in blood flow.

    Original languageEnglish (US)
    Pages (from-to)G399-G410
    JournalAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
    Volume284
    Issue number3 47-3
    StatePublished - Mar 1 2003

    Keywords

    • Gastric injury
    • Nitric oxide synthase inhibitors
    • Rats
    • Stomach

    ASJC Scopus subject areas

    • Physiology
    • Hepatology
    • Gastroenterology
    • Physiology (medical)

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  • Cite this

    West, S. D., Helmer, K. S., Chang, L. K., Cui, Y., Greeley, G. H., & Mercer, D. W. (2003). Cholecystokinin secretagogue-induced gastroprotection: Role of nitric oxide and blood flow. American Journal of Physiology - Gastrointestinal and Liver Physiology, 284(3 47-3), G399-G410.