Cholera toxin A subunit: Functional sites correlated with regions of secondary structure

Lawrence K. Duffy, Alexander Kurosky, Chun Yen Lai

    Research output: Contribution to journalArticle

    3 Citations (Scopus)

    Abstract

    The A subunit of cholera toxin contains the ADP-ribosyltransferase activity in its major constituent polypeptide A2 (Mr 23,000) which is responsible for the elevation of cAMP typically observed with most mammalian cell types after exposure to the toxin. The primary structure of the A subunit, recently established by sequence analyses, is presented and used as the basis for the secondary structure prediction according to the method of Chou and Fasman. The results indicated the presence of 27% α-helix, 25% β-structure, 12% β-turn, and 36% random coil. The majority of the β-structure consisted of six strands located in the NH2-terminal portion of the molecule (residues 33-106) covering one-half of the region corresponding to the A1 polypeptide portion. The β-sheet domain led immediately into the active site region characterized by the alternating structures of β-pleated sheet and α-helix (residues 95-140) similar to that reported for other NAD+ binding proteins. The presence of this structural feature in the region was confirmed by the use of another predictive method (J. Ganrier et al., J. Mol. Biol. 1978, 120, 97-120). In addition, two regions (residues 14-18 and 200-214), previously identified to contain binding sites for the B subunit as evidenced by chemical modification and monoclonal antibody studies, were found to be in α-helix configuration.

    Original languageEnglish (US)
    Pages (from-to)549-555
    Number of pages7
    JournalArchives of Biochemistry and Biophysics
    Volume239
    Issue number2
    DOIs
    StatePublished - 1985

    Fingerprint

    Cholera Toxin
    ADP Ribose Transferases
    Peptides
    Chemical modification
    varespladib methyl
    NAD
    Sequence Analysis
    Catalytic Domain
    Carrier Proteins
    Binding Sites
    Monoclonal Antibodies
    Cells
    Molecules

    ASJC Scopus subject areas

    • Biochemistry
    • Biophysics
    • Molecular Biology

    Cite this

    Cholera toxin A subunit : Functional sites correlated with regions of secondary structure. / Duffy, Lawrence K.; Kurosky, Alexander; Lai, Chun Yen.

    In: Archives of Biochemistry and Biophysics, Vol. 239, No. 2, 1985, p. 549-555.

    Research output: Contribution to journalArticle

    Duffy, Lawrence K. ; Kurosky, Alexander ; Lai, Chun Yen. / Cholera toxin A subunit : Functional sites correlated with regions of secondary structure. In: Archives of Biochemistry and Biophysics. 1985 ; Vol. 239, No. 2. pp. 549-555.
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