Cholera toxin-like toxin released by Salmonella species in the presence of mitomycin C

N. C. Molina, Johnny Peterson

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Several serotypes of Salmonella were shown to release increased amounts of a cholera toxin-like toxin during culture in vitro with mitomycin C (MTC). Filter-sterilized culture supernatants containing the toxin caused elongation of Chinese hamster ovary cells, which could be blocked by heating the supernatants at 100°C for 15 min or by adding mixed gangliosides or monospecific cholera antitoxin. When MTC was not added to the Salmonella cultures, little or no toxin was detected in crude, unconcentrated culture supernatants. Optimal production of toxin was observed in the presence of 0.5 μg of MTC per ml in shake flask cultures of Casamino Acids-yeast extract medium, Syncase, or peptone saline at 37°C. Meat infusion media (heart infusion and brain heart infusion) plus MTC resulted in poor toxin yield. Culture filtrates frequently could be diluted 1:8 and still result in elongation of Chinese hamster ovary cells.

Original languageEnglish
Pages (from-to)224-230
Number of pages7
JournalInfection and Immunity
Volume30
Issue number1
StatePublished - 1980

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Cholera Toxin
Mitomycin
Cricetulus
Salmonella
Ovary
Antitoxins
Peptones
Batch Cell Culture Techniques
Gangliosides
Cholera
Heating
Meat
Yeasts
salmonella toxin
Brain

ASJC Scopus subject areas

  • Immunology

Cite this

Cholera toxin-like toxin released by Salmonella species in the presence of mitomycin C. / Molina, N. C.; Peterson, Johnny.

In: Infection and Immunity, Vol. 30, No. 1, 1980, p. 224-230.

Research output: Contribution to journalArticle

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