Cholinergic and nitrergic regulation of in vivo giant migrating contractions in rat colon

Mona Li, Christopher P. Johnson, Mark B. Adams, Sushil K. Sarna

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

The aim of this study was to characterize in vivo rat colonic motor activity in normal and inflamed states and determine its neural regulation. Circular muscle contractions were recorded by surgically implanted strain-gauge transducers. The rat colon exhibited predominantly giant migrating contractions (GMCs) whose frequency decreased distally. Only a small percentage of these GMCs propagated in the distal direction; the rest occurred randomly. Phasic contractions were present, but their amplitude was very small compared with that of GMCs. Inflammation induced by oral administration of dextran sodium sulfate suppressed the frequency of GMCs in the proximal and middle but not in the distal colon. Frequency of GMCs was suppressed by intra-peritoneally administered atropine and 4-diphenylacetoxy-N-methyl-piperidine methiodide and was enhanced by N w-nitro-L-arginine methyl ester. Serotonin, tachykinin, and calcitonin gene-related peptide receptor or receptor subtype antagonists as well as guanethidine and suramin had no significant effect on the frequency of GMCs. Verapamil transiently suppressed the GMCs. In conclusion, unlike the canine and human colons, the rat colon exhibits frequent GMCs and their frequency is suppressed in inflammation. In vivo GMCs are stimulated by neural release of acetylcholine that acts on M 3 receptors. Constitutive release of nitric oxide may partially suppress their frequency.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
Volume283
Issue number3 46-3
StatePublished - Sep 2002

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Cholinergic Agents
Colon
Calcitonin Gene-Related Peptide Receptors
Inflammation
Guanethidine
Suramin
Tachykinins
Dextran Sulfate
Muscle Contraction
Verapamil
Transducers
Atropine
Acetylcholine
Oral Administration
Canidae
Serotonin
Nitric Oxide
Motor Activity

Keywords

  • Diarrhea
  • Enteric neurons
  • Inflammation
  • N -nitro-L-arginine methyl ester
  • Peristaltic reflex

ASJC Scopus subject areas

  • Gastroenterology
  • Physiology

Cite this

Cholinergic and nitrergic regulation of in vivo giant migrating contractions in rat colon. / Li, Mona; Johnson, Christopher P.; Adams, Mark B.; Sarna, Sushil K.

In: American Journal of Physiology - Gastrointestinal and Liver Physiology, Vol. 283, No. 3 46-3, 09.2002.

Research output: Contribution to journalArticle

Li, Mona ; Johnson, Christopher P. ; Adams, Mark B. ; Sarna, Sushil K. / Cholinergic and nitrergic regulation of in vivo giant migrating contractions in rat colon. In: American Journal of Physiology - Gastrointestinal and Liver Physiology. 2002 ; Vol. 283, No. 3 46-3.
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