Cholinergic control of nerve growth factor in adult rats: Evidence from cortical cholinergic deafferentation and chronic drug treatment

Steffen Roßner, Gitta Wörtwein, Zezong Gu, Juan Yu, Reinhard Schliebs, Volker Bigl, J. Regino Perez-Polo

    Research output: Contribution to journalArticle

    31 Citations (Scopus)

    Abstract

    It is well documented that nerve growth factor (NGF) plays an important role in maintaining functions of cholinergic basal forebrain neurons. In the present study, we tested the hypothesis that cholinergic activity controls NGF levels in cholinoceptive neurons of the cerebral cortex and hippocampus. To address that question, we used both cholinergic deafferentation of cerebral cortex and hippocampus by cholinergic immunolesion with 192IgG- saporin and chronic pharmacological treatment of sham-treated and immunolesioned rats with the cholinergic agonist pilocarpine and the cholinergic antagonist scopolamine. We observed an increase in NGF protein levels in the cortex and hippocampus after cholinergic immunolesions and also after muscarinic receptor blockade by chronic intracerebroventricular scopolamine infusion in sham-treated rats after 2 weeks. There was no further increase in the accumulation of NGF after scopolamine treatment of immunolesioned rats. Chronic infusion of pilocarpine had no effect on cortical and hippocampal NGF protein levels in sham-treated rats. In rats with cholinergic immunolesions, however, pilocarpine did prevent the lesion- induced accumulation of NGF. There was no effect of cholinergic lesion and drug treatment on cortical or hippocampal NGF mRNA levels, consistent with the importance of NGF retrograde transport as opposed to its de novo synthesis. This study provides strong evidence for the hypothesis that there is cholinergic control of cortical and hippocampal NGF protein but not mRNA levels in adult rats.

    Original languageEnglish (US)
    Pages (from-to)947-953
    Number of pages7
    JournalJournal of Neurochemistry
    Volume69
    Issue number3
    StatePublished - Sep 1997

    Fingerprint

    Drug therapy
    Nerve Growth Factor
    Cholinergic Agents
    Rats
    Pharmaceutical Preparations
    Pilocarpine
    Scopolamine Hydrobromide
    Hippocampus
    Therapeutics
    Cerebral Cortex
    Neurons
    Intraventricular Infusions
    Cholinergic Agonists
    Messenger RNA
    Proteins
    Cholinergic Antagonists
    Muscarinic Receptors
    Placebos
    Pharmacology

    Keywords

    • 192IgG-saporin
    • Immunolesion
    • Muscarinic
    • Nerve growth factor
    • Pilocarpine
    • Scopolamine

    ASJC Scopus subject areas

    • Biochemistry
    • Cellular and Molecular Neuroscience

    Cite this

    Roßner, S., Wörtwein, G., Gu, Z., Yu, J., Schliebs, R., Bigl, V., & Perez-Polo, J. R. (1997). Cholinergic control of nerve growth factor in adult rats: Evidence from cortical cholinergic deafferentation and chronic drug treatment. Journal of Neurochemistry, 69(3), 947-953.

    Cholinergic control of nerve growth factor in adult rats : Evidence from cortical cholinergic deafferentation and chronic drug treatment. / Roßner, Steffen; Wörtwein, Gitta; Gu, Zezong; Yu, Juan; Schliebs, Reinhard; Bigl, Volker; Perez-Polo, J. Regino.

    In: Journal of Neurochemistry, Vol. 69, No. 3, 09.1997, p. 947-953.

    Research output: Contribution to journalArticle

    Roßner, S, Wörtwein, G, Gu, Z, Yu, J, Schliebs, R, Bigl, V & Perez-Polo, JR 1997, 'Cholinergic control of nerve growth factor in adult rats: Evidence from cortical cholinergic deafferentation and chronic drug treatment', Journal of Neurochemistry, vol. 69, no. 3, pp. 947-953.
    Roßner, Steffen ; Wörtwein, Gitta ; Gu, Zezong ; Yu, Juan ; Schliebs, Reinhard ; Bigl, Volker ; Perez-Polo, J. Regino. / Cholinergic control of nerve growth factor in adult rats : Evidence from cortical cholinergic deafferentation and chronic drug treatment. In: Journal of Neurochemistry. 1997 ; Vol. 69, No. 3. pp. 947-953.
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