Chorioamniotic membrane senescence

A signal for parturition?

Faranak Behnia, Brandie D. Taylor, Michael Woodson, Marian Kacerovsky, Hal Hawkins, Stephen J. Fortunato, George Saade, Ramkumar Menon

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

Objective Senescence is an important biological phenomenon involved in both physiologic and pathologic processes. We propose that chorioamniotic membrane senescence is a mechanism associated with human parturition. The present study was conducted to explore the association between senescence and normal term parturition by examining the morphologic and biochemical evidences in chorioamniotic membranes. Study Design Chorioamniotic membranes were collected from normal term deliveries; group 1: term labor and group 2: term, not in labor. Senescence-related morphologic changes were determined by transmission electron microscopy and biochemical changes were studied by senescence-associated (SA) β-galactosidase staining. Amniotic fluid samples collected from both term labor and term not in labor were analyzed for 14 SA secretory phenotype (SASP) markers. Results Morphologic evidence of cellular senescence (enlarged cells and organelles) and a higher number of SA β-galactosidase-stained amnion and chorion cells were observed in chorioamniotic membranes obtained from women in labor at term, when compared to term not in labor. The concentration of proinflammatory SASP markers (granulocyte macrophage colony-stimulating factor, interleukin-6 and -8) was significantly higher in the amniotic fluid of women in labor at term than women not in labor. In contrast, SASP factors that protect against cell death (eotaxin-1, soluble Fas ligand, osteoprotegerin, and intercellular adhesion molecule-1) were significantly lower in the amniotic fluid samples from term labor. Conclusion Morphologic and biochemical features of senescence were more frequent in chorioamniotic membranes from women who experienced term labor. Senescence of chorioamniotic membranes were also associated with amniotic fluid SASP markers.

Original languageEnglish (US)
Pages (from-to)359e1-359e16
JournalAmerican Journal of Obstetrics and Gynecology
Volume213
Issue number3
DOIs
StatePublished - Sep 1 2015

Fingerprint

Parturition
Membranes
Amniotic Fluid
Galactosidases
Phenotype
Chemokine CCL11
Biological Phenomena
Chorion
Osteoprotegerin
Amnion
Fas Ligand Protein
Cell Aging
Pathologic Processes
Intercellular Adhesion Molecule-1
Granulocyte-Macrophage Colony-Stimulating Factor
Transmission Electron Microscopy
Interleukin-8
Organelles
Interleukin-6
Cell Death

Keywords

  • aging
  • delivery
  • fetal signals
  • labor
  • pregnancy
  • premature birth
  • senescence-associated secretory phenotype
  • sterile inflammation
  • β-galactosidase

ASJC Scopus subject areas

  • Obstetrics and Gynecology

Cite this

Behnia, F., Taylor, B. D., Woodson, M., Kacerovsky, M., Hawkins, H., Fortunato, S. J., ... Menon, R. (2015). Chorioamniotic membrane senescence: A signal for parturition? American Journal of Obstetrics and Gynecology, 213(3), 359e1-359e16. https://doi.org/10.1016/j.ajog.2015.05.041

Chorioamniotic membrane senescence : A signal for parturition? / Behnia, Faranak; Taylor, Brandie D.; Woodson, Michael; Kacerovsky, Marian; Hawkins, Hal; Fortunato, Stephen J.; Saade, George; Menon, Ramkumar.

In: American Journal of Obstetrics and Gynecology, Vol. 213, No. 3, 01.09.2015, p. 359e1-359e16.

Research output: Contribution to journalArticle

Behnia, F, Taylor, BD, Woodson, M, Kacerovsky, M, Hawkins, H, Fortunato, SJ, Saade, G & Menon, R 2015, 'Chorioamniotic membrane senescence: A signal for parturition?', American Journal of Obstetrics and Gynecology, vol. 213, no. 3, pp. 359e1-359e16. https://doi.org/10.1016/j.ajog.2015.05.041
Behnia F, Taylor BD, Woodson M, Kacerovsky M, Hawkins H, Fortunato SJ et al. Chorioamniotic membrane senescence: A signal for parturition? American Journal of Obstetrics and Gynecology. 2015 Sep 1;213(3):359e1-359e16. https://doi.org/10.1016/j.ajog.2015.05.041
Behnia, Faranak ; Taylor, Brandie D. ; Woodson, Michael ; Kacerovsky, Marian ; Hawkins, Hal ; Fortunato, Stephen J. ; Saade, George ; Menon, Ramkumar. / Chorioamniotic membrane senescence : A signal for parturition?. In: American Journal of Obstetrics and Gynecology. 2015 ; Vol. 213, No. 3. pp. 359e1-359e16.
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abstract = "Objective Senescence is an important biological phenomenon involved in both physiologic and pathologic processes. We propose that chorioamniotic membrane senescence is a mechanism associated with human parturition. The present study was conducted to explore the association between senescence and normal term parturition by examining the morphologic and biochemical evidences in chorioamniotic membranes. Study Design Chorioamniotic membranes were collected from normal term deliveries; group 1: term labor and group 2: term, not in labor. Senescence-related morphologic changes were determined by transmission electron microscopy and biochemical changes were studied by senescence-associated (SA) β-galactosidase staining. Amniotic fluid samples collected from both term labor and term not in labor were analyzed for 14 SA secretory phenotype (SASP) markers. Results Morphologic evidence of cellular senescence (enlarged cells and organelles) and a higher number of SA β-galactosidase-stained amnion and chorion cells were observed in chorioamniotic membranes obtained from women in labor at term, when compared to term not in labor. The concentration of proinflammatory SASP markers (granulocyte macrophage colony-stimulating factor, interleukin-6 and -8) was significantly higher in the amniotic fluid of women in labor at term than women not in labor. In contrast, SASP factors that protect against cell death (eotaxin-1, soluble Fas ligand, osteoprotegerin, and intercellular adhesion molecule-1) were significantly lower in the amniotic fluid samples from term labor. Conclusion Morphologic and biochemical features of senescence were more frequent in chorioamniotic membranes from women who experienced term labor. Senescence of chorioamniotic membranes were also associated with amniotic fluid SASP markers.",
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N2 - Objective Senescence is an important biological phenomenon involved in both physiologic and pathologic processes. We propose that chorioamniotic membrane senescence is a mechanism associated with human parturition. The present study was conducted to explore the association between senescence and normal term parturition by examining the morphologic and biochemical evidences in chorioamniotic membranes. Study Design Chorioamniotic membranes were collected from normal term deliveries; group 1: term labor and group 2: term, not in labor. Senescence-related morphologic changes were determined by transmission electron microscopy and biochemical changes were studied by senescence-associated (SA) β-galactosidase staining. Amniotic fluid samples collected from both term labor and term not in labor were analyzed for 14 SA secretory phenotype (SASP) markers. Results Morphologic evidence of cellular senescence (enlarged cells and organelles) and a higher number of SA β-galactosidase-stained amnion and chorion cells were observed in chorioamniotic membranes obtained from women in labor at term, when compared to term not in labor. The concentration of proinflammatory SASP markers (granulocyte macrophage colony-stimulating factor, interleukin-6 and -8) was significantly higher in the amniotic fluid of women in labor at term than women not in labor. In contrast, SASP factors that protect against cell death (eotaxin-1, soluble Fas ligand, osteoprotegerin, and intercellular adhesion molecule-1) were significantly lower in the amniotic fluid samples from term labor. Conclusion Morphologic and biochemical features of senescence were more frequent in chorioamniotic membranes from women who experienced term labor. Senescence of chorioamniotic membranes were also associated with amniotic fluid SASP markers.

AB - Objective Senescence is an important biological phenomenon involved in both physiologic and pathologic processes. We propose that chorioamniotic membrane senescence is a mechanism associated with human parturition. The present study was conducted to explore the association between senescence and normal term parturition by examining the morphologic and biochemical evidences in chorioamniotic membranes. Study Design Chorioamniotic membranes were collected from normal term deliveries; group 1: term labor and group 2: term, not in labor. Senescence-related morphologic changes were determined by transmission electron microscopy and biochemical changes were studied by senescence-associated (SA) β-galactosidase staining. Amniotic fluid samples collected from both term labor and term not in labor were analyzed for 14 SA secretory phenotype (SASP) markers. Results Morphologic evidence of cellular senescence (enlarged cells and organelles) and a higher number of SA β-galactosidase-stained amnion and chorion cells were observed in chorioamniotic membranes obtained from women in labor at term, when compared to term not in labor. The concentration of proinflammatory SASP markers (granulocyte macrophage colony-stimulating factor, interleukin-6 and -8) was significantly higher in the amniotic fluid of women in labor at term than women not in labor. In contrast, SASP factors that protect against cell death (eotaxin-1, soluble Fas ligand, osteoprotegerin, and intercellular adhesion molecule-1) were significantly lower in the amniotic fluid samples from term labor. Conclusion Morphologic and biochemical features of senescence were more frequent in chorioamniotic membranes from women who experienced term labor. Senescence of chorioamniotic membranes were also associated with amniotic fluid SASP markers.

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