Choroidal γδ T cells in protection against retinal pigment epithelium and retinal injury

Zhenyang Zhao, Yuejin Liang, Yin Liu, Pei Xu, Miles J. Flamme-Wiese, Deming Sun, Jiaren Sun, Robert F. Mullins, Yan Chen, Jiyang Cai

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

γδ T cells located near the epithelial barrier are integral components of local inflammatory and innate immune responses. We have previously reported the presence of choroidal γδ T cells in a model of chronic degeneration of the retinal pigment epithelium (RPE). The goals of the current study were to further define the functions of choroidal γδ T cells and to explore the underlying mechanisms of their action. Our data demonstrate that choroidal γδ T cells are activated by RPE injury in response to NaIO3 treatment, and that they express genes that encode immunosuppressive cytokines, such as IL-4 and IL-10. γδ-T-cell-deficient mice developed profound RPE and retinal damage at doses that caused minimal effects in wild-type mice, and adoptive transfer of γδ T cells prevented sensitization. Intravitreal injection of IL-4 and IL-10 ameliorated RPE toxicity that was induced by NaIO3Ex vivo coculture of γδ T cells with RPE explants activated the production of anti-inflammatory cytokines via an aryl hydrocarbon receptor (AhR)-dependent mechanism. AhR deficiency abolished the protective effects of γδ T cells after adoptive transfer. Collectively, these findings define important roles for choroid γδ T cells in maintaining tissue homeostasis in the outer retina.-Zhao, Z., Liang, Y., Liu, Y., Xu, P., Flamme-Wiese, M. J., Sun, D., Sun, J., Mullins, R. F., Chen, Y., Cai, J. Choroidal γδ T cells in protection against retinal pigment epithelium and retinal injury.

Original languageEnglish (US)
Pages (from-to)4903-4916
Number of pages14
JournalFASEB journal : official publication of the Federation of American Societies for Experimental Biology
Volume31
Issue number11
DOIs
StatePublished - Nov 1 2017

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Retinal Pigments
Cytoprotection
T-cells
Retinal Pigment Epithelium
T-Lymphocytes
Wounds and Injuries
Aryl Hydrocarbon Receptors
Adoptive Transfer
Solar System
Interleukin-4
Sun
Interleukin-10
Tissue homeostasis
Cytokines
Intravitreal Injections
Choroid
Immunosuppressive Agents
Coculture Techniques
Innate Immunity
Toxicity

Keywords

  • AhR
  • choriocapillaris drop out
  • immune modulation
  • inflammation

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

Choroidal γδ T cells in protection against retinal pigment epithelium and retinal injury. / Zhao, Zhenyang; Liang, Yuejin; Liu, Yin; Xu, Pei; Flamme-Wiese, Miles J.; Sun, Deming; Sun, Jiaren; Mullins, Robert F.; Chen, Yan; Cai, Jiyang.

In: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 31, No. 11, 01.11.2017, p. 4903-4916.

Research output: Contribution to journalArticle

Zhao, Zhenyang ; Liang, Yuejin ; Liu, Yin ; Xu, Pei ; Flamme-Wiese, Miles J. ; Sun, Deming ; Sun, Jiaren ; Mullins, Robert F. ; Chen, Yan ; Cai, Jiyang. / Choroidal γδ T cells in protection against retinal pigment epithelium and retinal injury. In: FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 2017 ; Vol. 31, No. 11. pp. 4903-4916.
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AU - Sun, Deming

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