Chromosomal DNA contains the gene coding for Salmonella enterotoxin

Ashok Chopra, C. W. Houston, Johnny Peterson, J. J. Mekalanos

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

This report examines the genetic basis for Salmonella enterotoxin production. The examination was conducted using an internal XbaI/HincII sequence of plasmid pJM17 encoding cholera toxin as a gene probe (ctxAB). This gene probe detected some sequence homology with the total as well as digested chromosomal DNA fragments from Salmonella strains under reduced stringent conditions in dot-blot and Southern-blot hybridization experiments. Our hybridization analysis results suggested that the enterotoxin (ent) gene of Salmonella resides on chromosomal DNA and that the Salmonella ent operon might be duplicated on the Salmonella chromosome.

Original languageEnglish (US)
Pages (from-to)345-349
Number of pages5
JournalFEMS Microbiology Letters
Volume43
Issue number3
StatePublished - 1987

Fingerprint

Salmonella
DNA
Genes
Cholera Toxin
Operon
Sequence Homology
Southern Blotting
Plasmids
Chromosomes
Salmonella enterotoxin

Keywords

  • (Southern-blot hybridization)
  • cholera toxin
  • chromosomal gene
  • Salmonella enterotoxin

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

Chromosomal DNA contains the gene coding for Salmonella enterotoxin. / Chopra, Ashok; Houston, C. W.; Peterson, Johnny; Mekalanos, J. J.

In: FEMS Microbiology Letters, Vol. 43, No. 3, 1987, p. 345-349.

Research output: Contribution to journalArticle

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