Chromosome Orientation fluorescence in situ hybridization or strand-specific FISH.

Susan M. Bailey, Eli S. Williams, Michael Cornforth, Edwin H. Goodwin

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Chromosome Orientation FISH (CO-FISH) is a technique that can be used to extend the information obtainable from standard FISH to include the relative orientation of two or more DNA sequences within a chromosome. CO-FISH can determine the absolute 5'-to-3' direction of a DNA sequence relative to the short arm-to-long arm axis of the chromosome, and so was originally termed "COD-FISH" (Chromosome Orientation and Direction FISH). CO-FISH has been employed to detect chromosomal inversions associated with isochromosome formation, various pericentric inversions, and to confirm the origin of lateral asymmetry. More recent and sophisticated applications of CO-FISH include distinction between telomeres produced via leading- vs. lagging-strand DNA synthesis, identification of interstitial blocks of telomere sequence that result from inappropriate fusion to double-strand breaks (telomere-DSB fusion), discovery of elevated rates of mitotic recombination at chromosomal termini and sister chromatid exchange within telomeric DNA (T-SCE), establishing replication timing of mammalian telomeres throughout S-phase (ReD-FISH) and to identify chromosomes, in combination with spectral karyotyping (SKY-CO-FISH).

Original languageEnglish (US)
Pages (from-to)173-183
Number of pages11
JournalMethods in molecular biology (Clifton, N.J.)
Volume659
StatePublished - 2010
Externally publishedYes

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Fluorescence In Situ Hybridization
Chromosomes
Telomere
Spectral Karyotyping
Isochromosomes
Sister Chromatid Exchange
S Phase
Genetic Recombination
DNA

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Chromosome Orientation fluorescence in situ hybridization or strand-specific FISH. / Bailey, Susan M.; Williams, Eli S.; Cornforth, Michael; Goodwin, Edwin H.

In: Methods in molecular biology (Clifton, N.J.), Vol. 659, 2010, p. 173-183.

Research output: Contribution to journalArticle

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AU - Williams, Eli S.

AU - Cornforth, Michael

AU - Goodwin, Edwin H.

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AB - Chromosome Orientation FISH (CO-FISH) is a technique that can be used to extend the information obtainable from standard FISH to include the relative orientation of two or more DNA sequences within a chromosome. CO-FISH can determine the absolute 5'-to-3' direction of a DNA sequence relative to the short arm-to-long arm axis of the chromosome, and so was originally termed "COD-FISH" (Chromosome Orientation and Direction FISH). CO-FISH has been employed to detect chromosomal inversions associated with isochromosome formation, various pericentric inversions, and to confirm the origin of lateral asymmetry. More recent and sophisticated applications of CO-FISH include distinction between telomeres produced via leading- vs. lagging-strand DNA synthesis, identification of interstitial blocks of telomere sequence that result from inappropriate fusion to double-strand breaks (telomere-DSB fusion), discovery of elevated rates of mitotic recombination at chromosomal termini and sister chromatid exchange within telomeric DNA (T-SCE), establishing replication timing of mammalian telomeres throughout S-phase (ReD-FISH) and to identify chromosomes, in combination with spectral karyotyping (SKY-CO-FISH).

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