Chronic binge alcohol exposure during pregnancy impairs rat maternal uterine vascular function

Kaviarasan Subramanian, Vishal D. Naik, Kunju Sathishkumar, Chandrashekar Yallampalli, George Saade, Gary Hankins, Jayanth Ramadoss

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Background: Alcohol exposure during pregnancy results in an array of structural and functional abnormalities called fetal alcohol spectrum disorders (FASD). Alcohol dysregulates the exquisite coordination and regulation of gestational adaptations at the level of the uterine vasculature. We herein hypothesized that chronic binge-like alcohol results in uterine vascular dysfunction and impairs maternal uterine artery reactivity to vasoconstrictors and dilators. Methods: We utilized a once-daily binge alcohol (4.5 g/kg body weight) exposure paradigm (gestational day 7 to 17) in a pregnant rat model system and investigated primary uterine artery function in response to vasoconstrictors and vasodilators utilizing wire myography. Results: Alcohol (peak blood alcohol concentration, 216 mg/dl) produced uterine vascular dysfunction. Alcohol did not produce altered uterine vascular reactivity to α1 adrenergic agonist phenylephrine or the prostanoid thromboxane. However, alcohol specifically impaired acetylcholine (ACh)-mediated uterine artery vasodilation but exogenous endothelium-independent vasodilators like sodium nitroprusside exhibited no alcohol effect; ACh significantly decreased vessel relaxation (p = 0.003; ↓pD2 [negative log molar ACh concentration producing the half maximum response], -7.004 ± 0.215 vs. -6.310 ± 0.208; EMax [maximal ACh response], 92% vs. 75%). Conclusions: We conclude that moderate alcohol exposure impairs uterine vascular function in pregnant mothers. Alcohol specifically impairs agonist-induced uterine artery vasodilation. In summary, the maternal uterine compartment may play a significant role in the pathogenesis of FASD. Thus, the mechanistic targets of alcohol at the level of both the mother and the fetus need to be considered in order to develop effective therapeutic treatment strategies for FASD.

Original languageEnglish
Pages (from-to)1832-1838
Number of pages7
JournalAlcoholism: Clinical and Experimental Research
Volume38
Issue number7
DOIs
StatePublished - 2014

Fingerprint

Blood Vessels
Rats
Alcohols
Mothers
Pregnancy
Uterine Artery
Fetal Alcohol Spectrum Disorders
Acetylcholine
Vasoconstrictor Agents
Vasodilator Agents
Vasodilation
Myography
Adrenergic Agonists
Thromboxanes
Nitroprusside
Phenylephrine
Prostaglandins
Endothelium
Fetus
Body Weight

Keywords

  • Alcohol
  • Endothelium
  • Fetal alcohol spectrum disorders
  • Maternal
  • Uterine
  • Vascular

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Psychiatry and Mental health
  • Toxicology
  • Medicine(all)

Cite this

Chronic binge alcohol exposure during pregnancy impairs rat maternal uterine vascular function. / Subramanian, Kaviarasan; Naik, Vishal D.; Sathishkumar, Kunju; Yallampalli, Chandrashekar; Saade, George; Hankins, Gary; Ramadoss, Jayanth.

In: Alcoholism: Clinical and Experimental Research, Vol. 38, No. 7, 2014, p. 1832-1838.

Research output: Contribution to journalArticle

Subramanian, K, Naik, VD, Sathishkumar, K, Yallampalli, C, Saade, G, Hankins, G & Ramadoss, J 2014, 'Chronic binge alcohol exposure during pregnancy impairs rat maternal uterine vascular function', Alcoholism: Clinical and Experimental Research, vol. 38, no. 7, pp. 1832-1838. https://doi.org/10.1111/acer.12431
Subramanian, Kaviarasan ; Naik, Vishal D. ; Sathishkumar, Kunju ; Yallampalli, Chandrashekar ; Saade, George ; Hankins, Gary ; Ramadoss, Jayanth. / Chronic binge alcohol exposure during pregnancy impairs rat maternal uterine vascular function. In: Alcoholism: Clinical and Experimental Research. 2014 ; Vol. 38, No. 7. pp. 1832-1838.
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AU - Saade, George

AU - Hankins, Gary

AU - Ramadoss, Jayanth

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N2 - Background: Alcohol exposure during pregnancy results in an array of structural and functional abnormalities called fetal alcohol spectrum disorders (FASD). Alcohol dysregulates the exquisite coordination and regulation of gestational adaptations at the level of the uterine vasculature. We herein hypothesized that chronic binge-like alcohol results in uterine vascular dysfunction and impairs maternal uterine artery reactivity to vasoconstrictors and dilators. Methods: We utilized a once-daily binge alcohol (4.5 g/kg body weight) exposure paradigm (gestational day 7 to 17) in a pregnant rat model system and investigated primary uterine artery function in response to vasoconstrictors and vasodilators utilizing wire myography. Results: Alcohol (peak blood alcohol concentration, 216 mg/dl) produced uterine vascular dysfunction. Alcohol did not produce altered uterine vascular reactivity to α1 adrenergic agonist phenylephrine or the prostanoid thromboxane. However, alcohol specifically impaired acetylcholine (ACh)-mediated uterine artery vasodilation but exogenous endothelium-independent vasodilators like sodium nitroprusside exhibited no alcohol effect; ACh significantly decreased vessel relaxation (p = 0.003; ↓pD2 [negative log molar ACh concentration producing the half maximum response], -7.004 ± 0.215 vs. -6.310 ± 0.208; EMax [maximal ACh response], 92% vs. 75%). Conclusions: We conclude that moderate alcohol exposure impairs uterine vascular function in pregnant mothers. Alcohol specifically impairs agonist-induced uterine artery vasodilation. In summary, the maternal uterine compartment may play a significant role in the pathogenesis of FASD. Thus, the mechanistic targets of alcohol at the level of both the mother and the fetus need to be considered in order to develop effective therapeutic treatment strategies for FASD.

AB - Background: Alcohol exposure during pregnancy results in an array of structural and functional abnormalities called fetal alcohol spectrum disorders (FASD). Alcohol dysregulates the exquisite coordination and regulation of gestational adaptations at the level of the uterine vasculature. We herein hypothesized that chronic binge-like alcohol results in uterine vascular dysfunction and impairs maternal uterine artery reactivity to vasoconstrictors and dilators. Methods: We utilized a once-daily binge alcohol (4.5 g/kg body weight) exposure paradigm (gestational day 7 to 17) in a pregnant rat model system and investigated primary uterine artery function in response to vasoconstrictors and vasodilators utilizing wire myography. Results: Alcohol (peak blood alcohol concentration, 216 mg/dl) produced uterine vascular dysfunction. Alcohol did not produce altered uterine vascular reactivity to α1 adrenergic agonist phenylephrine or the prostanoid thromboxane. However, alcohol specifically impaired acetylcholine (ACh)-mediated uterine artery vasodilation but exogenous endothelium-independent vasodilators like sodium nitroprusside exhibited no alcohol effect; ACh significantly decreased vessel relaxation (p = 0.003; ↓pD2 [negative log molar ACh concentration producing the half maximum response], -7.004 ± 0.215 vs. -6.310 ± 0.208; EMax [maximal ACh response], 92% vs. 75%). Conclusions: We conclude that moderate alcohol exposure impairs uterine vascular function in pregnant mothers. Alcohol specifically impairs agonist-induced uterine artery vasodilation. In summary, the maternal uterine compartment may play a significant role in the pathogenesis of FASD. Thus, the mechanistic targets of alcohol at the level of both the mother and the fetus need to be considered in order to develop effective therapeutic treatment strategies for FASD.

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