Chronic hyperbaric oxygen treatment elicits an anti-oxidant response and attenuates atherosclerosis in apoE knockout mice

Bhalchandra J. Kudchodkar, Anson Pierce, Ladislav Dory

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

We previously demonstrated that hyperbaric oxygen (HBO) treatment inhibits diet-induced atherosclerosis in New Zealand White rabbits. In the present study we investigate the mechanisms that might be involved in the athero-protective effect of HBO treatment in a well-accepted model of atherosclerosis, the apoE knockout (KO) mouse. We examine the effects of daily HBO treatment (for 5 and 10 weeks) on the components of the anti-oxidant defense mechanism and the redox state in blood, liver and aortic tissues and compare them to those of untreated apoE KO mice. HBO treatment results in a significant reduction of aortic cholesterol content and decreased fatty streak formation. These changes are accompanied by a significant reduction of autoantibodies against oxidatively modified LDL and profound changes in the redox state of the liver and aortic tissues. A 10-week treatment significantly reduces hepatic levels of TBARS and oxidized glutathione, while significantly increases the levels of reduced glutathione, glutathione reductase (GR), transferase, Se-dependent glutathione peroxidase and catalase (CAT). The effects of HBO treatment are similar in the aortic tissues. These observations provide evidence that HBO treatment has a powerful effect on the redox state of relevant tissues and produces an environment that inhibits oxidation. The anti-oxidant response may be the key to the anti-atherogenic effect of HBO treatment.

Original languageEnglish (US)
Pages (from-to)28-35
Number of pages8
JournalAtherosclerosis
Volume193
Issue number1
DOIs
StatePublished - Jul 2007
Externally publishedYes

Fingerprint

Apolipoproteins E
Oxidants
Knockout Mice
Atherosclerosis
Oxygen
Oxidation-Reduction
Liver
Glutathione Disulfide
Glutathione Reductase
Glutathione Peroxidase
Glutathione Transferase
Autoantibodies
Catalase
Glutathione
Cholesterol
Diet
Rabbits

Keywords

  • Anti-oxidant enzymes
  • Aorta
  • Apoe knock-out mice
  • Atherosclerosis
  • Hyperbaric oxygen
  • Liver
  • Oxidative stress

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Chronic hyperbaric oxygen treatment elicits an anti-oxidant response and attenuates atherosclerosis in apoE knockout mice. / Kudchodkar, Bhalchandra J.; Pierce, Anson; Dory, Ladislav.

In: Atherosclerosis, Vol. 193, No. 1, 07.2007, p. 28-35.

Research output: Contribution to journalArticle

Kudchodkar, Bhalchandra J. ; Pierce, Anson ; Dory, Ladislav. / Chronic hyperbaric oxygen treatment elicits an anti-oxidant response and attenuates atherosclerosis in apoE knockout mice. In: Atherosclerosis. 2007 ; Vol. 193, No. 1. pp. 28-35.
@article{c2ae4c94821247f0b9698f59c0d38fae,
title = "Chronic hyperbaric oxygen treatment elicits an anti-oxidant response and attenuates atherosclerosis in apoE knockout mice",
abstract = "We previously demonstrated that hyperbaric oxygen (HBO) treatment inhibits diet-induced atherosclerosis in New Zealand White rabbits. In the present study we investigate the mechanisms that might be involved in the athero-protective effect of HBO treatment in a well-accepted model of atherosclerosis, the apoE knockout (KO) mouse. We examine the effects of daily HBO treatment (for 5 and 10 weeks) on the components of the anti-oxidant defense mechanism and the redox state in blood, liver and aortic tissues and compare them to those of untreated apoE KO mice. HBO treatment results in a significant reduction of aortic cholesterol content and decreased fatty streak formation. These changes are accompanied by a significant reduction of autoantibodies against oxidatively modified LDL and profound changes in the redox state of the liver and aortic tissues. A 10-week treatment significantly reduces hepatic levels of TBARS and oxidized glutathione, while significantly increases the levels of reduced glutathione, glutathione reductase (GR), transferase, Se-dependent glutathione peroxidase and catalase (CAT). The effects of HBO treatment are similar in the aortic tissues. These observations provide evidence that HBO treatment has a powerful effect on the redox state of relevant tissues and produces an environment that inhibits oxidation. The anti-oxidant response may be the key to the anti-atherogenic effect of HBO treatment.",
keywords = "Anti-oxidant enzymes, Aorta, Apoe knock-out mice, Atherosclerosis, Hyperbaric oxygen, Liver, Oxidative stress",
author = "Kudchodkar, {Bhalchandra J.} and Anson Pierce and Ladislav Dory",
year = "2007",
month = "7",
doi = "10.1016/j.atherosclerosis.2006.08.018",
language = "English (US)",
volume = "193",
pages = "28--35",
journal = "Atherosclerosis",
issn = "0021-9150",
publisher = "Elsevier Ireland Ltd",
number = "1",

}

TY - JOUR

T1 - Chronic hyperbaric oxygen treatment elicits an anti-oxidant response and attenuates atherosclerosis in apoE knockout mice

AU - Kudchodkar, Bhalchandra J.

AU - Pierce, Anson

AU - Dory, Ladislav

PY - 2007/7

Y1 - 2007/7

N2 - We previously demonstrated that hyperbaric oxygen (HBO) treatment inhibits diet-induced atherosclerosis in New Zealand White rabbits. In the present study we investigate the mechanisms that might be involved in the athero-protective effect of HBO treatment in a well-accepted model of atherosclerosis, the apoE knockout (KO) mouse. We examine the effects of daily HBO treatment (for 5 and 10 weeks) on the components of the anti-oxidant defense mechanism and the redox state in blood, liver and aortic tissues and compare them to those of untreated apoE KO mice. HBO treatment results in a significant reduction of aortic cholesterol content and decreased fatty streak formation. These changes are accompanied by a significant reduction of autoantibodies against oxidatively modified LDL and profound changes in the redox state of the liver and aortic tissues. A 10-week treatment significantly reduces hepatic levels of TBARS and oxidized glutathione, while significantly increases the levels of reduced glutathione, glutathione reductase (GR), transferase, Se-dependent glutathione peroxidase and catalase (CAT). The effects of HBO treatment are similar in the aortic tissues. These observations provide evidence that HBO treatment has a powerful effect on the redox state of relevant tissues and produces an environment that inhibits oxidation. The anti-oxidant response may be the key to the anti-atherogenic effect of HBO treatment.

AB - We previously demonstrated that hyperbaric oxygen (HBO) treatment inhibits diet-induced atherosclerosis in New Zealand White rabbits. In the present study we investigate the mechanisms that might be involved in the athero-protective effect of HBO treatment in a well-accepted model of atherosclerosis, the apoE knockout (KO) mouse. We examine the effects of daily HBO treatment (for 5 and 10 weeks) on the components of the anti-oxidant defense mechanism and the redox state in blood, liver and aortic tissues and compare them to those of untreated apoE KO mice. HBO treatment results in a significant reduction of aortic cholesterol content and decreased fatty streak formation. These changes are accompanied by a significant reduction of autoantibodies against oxidatively modified LDL and profound changes in the redox state of the liver and aortic tissues. A 10-week treatment significantly reduces hepatic levels of TBARS and oxidized glutathione, while significantly increases the levels of reduced glutathione, glutathione reductase (GR), transferase, Se-dependent glutathione peroxidase and catalase (CAT). The effects of HBO treatment are similar in the aortic tissues. These observations provide evidence that HBO treatment has a powerful effect on the redox state of relevant tissues and produces an environment that inhibits oxidation. The anti-oxidant response may be the key to the anti-atherogenic effect of HBO treatment.

KW - Anti-oxidant enzymes

KW - Aorta

KW - Apoe knock-out mice

KW - Atherosclerosis

KW - Hyperbaric oxygen

KW - Liver

KW - Oxidative stress

UR - http://www.scopus.com/inward/record.url?scp=34248678425&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34248678425&partnerID=8YFLogxK

U2 - 10.1016/j.atherosclerosis.2006.08.018

DO - 10.1016/j.atherosclerosis.2006.08.018

M3 - Article

VL - 193

SP - 28

EP - 35

JO - Atherosclerosis

JF - Atherosclerosis

SN - 0021-9150

IS - 1

ER -