Abstract
This study examined the effects of low frequency chronic stimulation on expression of the mRNA encoding the two isoforms of the α1 subunit of the dihydropyridine receptor (DHPR) calcium channel, a critical component of skeletal muscle excitation-contraction coupling. RNase protection assay was used to determine alteration in isoform expression in 5-day, 9-day and 13-day chronically stimulated rat tibialis anterior muscle, and to compare it with soleus and extensor digitorum longus muscles. Low frequency chronic stimulation was associated not only with a significant decrease in the mRNA level of the skeletal isoform of the DHPR, but also with a significant increase in the mRNA level of the cardiac isoform of the DHPR, the overwhelming majority of which was the adult splice variant. Significant levels of cardiac DHPR mRNA expression were also found in normal adult slow twitch soleus muscle. These findings raise the question of a potential role for the cardiac DHPR in certain adult skeletal muscles.
| Original language | English |
|---|---|
| Pages (from-to) | 217-222 |
| Number of pages | 6 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 235 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jun 9 1997 |
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ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
Chronic stimulation differentially modulates expression of mRNA for dihydropyridine receptor isoforms in rat fast twitch skeletal muscle. / Péréon, Yann; Navarro, Javier; Hamilton, Marc; Booth, Frank W.; Palade, Philip.
In: Biochemical and Biophysical Research Communications, Vol. 235, No. 1, 09.06.1997, p. 217-222.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Chronic stimulation differentially modulates expression of mRNA for dihydropyridine receptor isoforms in rat fast twitch skeletal muscle
AU - Péréon, Yann
AU - Navarro, Javier
AU - Hamilton, Marc
AU - Booth, Frank W.
AU - Palade, Philip
PY - 1997/6/9
Y1 - 1997/6/9
N2 - This study examined the effects of low frequency chronic stimulation on expression of the mRNA encoding the two isoforms of the α1 subunit of the dihydropyridine receptor (DHPR) calcium channel, a critical component of skeletal muscle excitation-contraction coupling. RNase protection assay was used to determine alteration in isoform expression in 5-day, 9-day and 13-day chronically stimulated rat tibialis anterior muscle, and to compare it with soleus and extensor digitorum longus muscles. Low frequency chronic stimulation was associated not only with a significant decrease in the mRNA level of the skeletal isoform of the DHPR, but also with a significant increase in the mRNA level of the cardiac isoform of the DHPR, the overwhelming majority of which was the adult splice variant. Significant levels of cardiac DHPR mRNA expression were also found in normal adult slow twitch soleus muscle. These findings raise the question of a potential role for the cardiac DHPR in certain adult skeletal muscles.
AB - This study examined the effects of low frequency chronic stimulation on expression of the mRNA encoding the two isoforms of the α1 subunit of the dihydropyridine receptor (DHPR) calcium channel, a critical component of skeletal muscle excitation-contraction coupling. RNase protection assay was used to determine alteration in isoform expression in 5-day, 9-day and 13-day chronically stimulated rat tibialis anterior muscle, and to compare it with soleus and extensor digitorum longus muscles. Low frequency chronic stimulation was associated not only with a significant decrease in the mRNA level of the skeletal isoform of the DHPR, but also with a significant increase in the mRNA level of the cardiac isoform of the DHPR, the overwhelming majority of which was the adult splice variant. Significant levels of cardiac DHPR mRNA expression were also found in normal adult slow twitch soleus muscle. These findings raise the question of a potential role for the cardiac DHPR in certain adult skeletal muscles.
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U2 - 10.1006/bbrc.1997.6753
DO - 10.1006/bbrc.1997.6753
M3 - Article
VL - 235
SP - 217
EP - 222
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -