Cigarette smoke condensate enhances respiratory syncytial virus-induced chemokine release by modulating NF-kappa B and interferon regulatory factor activation

Shawn Monique Castro, Deepthi Kolli, Antonieta Guerrero-Plata, Roberto Garofalo, Antonella Casola

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Exposure to cigarette smoke is a risk factor contributing to the severity of respiratory tract infections associated with respiratory syncytial virus (RSV). Stimulation of airway epithelial cells by either RSV or cigarette smoke condensate (CSC) has been shown to induce secretion of the proinflammatory chemokines. However, the effect of coexposure of airway epithelial cells to CSC and RSV on inducible chemokine production has not been previously investigated. The results of this study indicate that CSC costimulation significantly increased RSV-induced interleukin-8 (IL-8) and monocyte chemoattactant protein-1 gene and protein expression when compared with each stimulus alone. Promoter deletion studies identified the interferon stimulatory response element (ISRE) of the IL-8 promoter as a critical region responsible for the synergistic increase of IL-8 gene transcription during mixed exposure. CSC costimulation enhanced RSV-induced activation of interferon regulatory factor (IRF)-1 and IRF-7, which bind to the ISRE site. CSC also furthered RSV-induced activation of the transcription factor nuclear factor kappa B (NF-κB), as shown by increased NF-κB DNA binding to its specific site of the IL-8 promoter and increased NF-κB-driven gene transcription. Therefore, our data demonstrate that a combined exposure to CSC and RSV synergistically increases chemokine expression in airway epithelial cells, suggesting that CSC contributes to an exuberant immune response to RSV by stimulating overlapping signal transduction pathways.

Original languageEnglish (US)
Pages (from-to)509-518
Number of pages10
JournalToxicological Sciences
Volume106
Issue number2
DOIs
StatePublished - 2008

Fingerprint

Interferon Regulatory Factors
Respiratory Syncytial Viruses
NF-kappa B
Viruses
Chemokines
Smoke
Tobacco Products
Chemical activation
Interleukin-8
Virus Activation
Epithelial Cells
Response Elements
Transcription
Interferons
Interferon Regulatory Factor-7
Genes
Interferon Regulatory Factor-1
Signal transduction
Respiratory Tract Infections
Monocytes

Keywords

  • Chemokines
  • Cigarette smoke condensate
  • IRF
  • NF-κB
  • RSV

ASJC Scopus subject areas

  • Toxicology

Cite this

Cigarette smoke condensate enhances respiratory syncytial virus-induced chemokine release by modulating NF-kappa B and interferon regulatory factor activation. / Castro, Shawn Monique; Kolli, Deepthi; Guerrero-Plata, Antonieta; Garofalo, Roberto; Casola, Antonella.

In: Toxicological Sciences, Vol. 106, No. 2, 2008, p. 509-518.

Research output: Contribution to journalArticle

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