Circadian rhythms in gastrin receptors in rat fundic stomach

Norma H. Rubin, Pomila Singh, Gunnar Alinder, George H. Greeley, Phillip L. Rayford, WOP J. Rietveld, James C. Thompson

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Circadian rhythmicity in the number of gastrin receptors in rat fundic mucosa was characterized and was related to the concentrations of gastrin in serum and in antrum. Male Sprague-Dawley rats were acclimated to 12 hr light alternating with 12 hr darkness. Subgroups of six rats each were killed at 4-hr intervals. Fundic mucosa was collected for measurement of gastrin receptors; serum and antral tissues were collected for measurement of gastrin levels by radioimmunoassay. Circadian periodicity in the data was determined by cosinor analyses. In both freely fed and fasted rats, gastrin receptors showed circadian variation (range 2.5-10 fmol/mg protein), as did serum gastrin concentrations (range in fed rats 195-407 pg/ml). The phasing of the intrinsic circadian variation in gastrin receptor level that was observed in the fasted rats was advanced by a few hours in fed rats. This shift is probably due to food-induced gastrin release, resulting in gastrin-mediated down-regulation of gastrin receptors, followed by up-regulation of gastrin receptors. Food-related effects were thus superimposed upon the intrinsic circadian rhythms in gastrin receptor levels, causing the circadian variation in gastrin receptor levels in the fed rats to be shifted forward compared to that in the fasted rats. No significant circadian rhythms, on the other other hand, were found in concentrations of gastrin in the antrum. These results suggest that changes in sensitivity of target tissues to hormones are related to both intrinsic circadian rhythms in levels of hormone receptors and also to food-related changes in hormone-receptor levels mediated by changing serum hormone levels.

Original languageEnglish
Pages (from-to)931-937
Number of pages7
JournalDigestive Diseases and Sciences
Volume33
Issue number8
DOIs
StatePublished - Aug 1988

Fingerprint

Cholecystokinin B Receptor
Circadian Rhythm
Stomach
Gastrins
Hormones
Periodicity
Food
Mucous Membrane
Serum
Darkness
Radioimmunoassay
Sprague Dawley Rats
Blood Proteins
Up-Regulation
Down-Regulation
Hand
Light

Keywords

  • circadian rhythms
  • gastrin
  • gastrin receptors
  • rat
  • stomach

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Rubin, N. H., Singh, P., Alinder, G., Greeley, G. H., Rayford, P. L., Rietveld, WOP. J., & Thompson, J. C. (1988). Circadian rhythms in gastrin receptors in rat fundic stomach. Digestive Diseases and Sciences, 33(8), 931-937. https://doi.org/10.1007/BF01535987

Circadian rhythms in gastrin receptors in rat fundic stomach. / Rubin, Norma H.; Singh, Pomila; Alinder, Gunnar; Greeley, George H.; Rayford, Phillip L.; Rietveld, WOP J.; Thompson, James C.

In: Digestive Diseases and Sciences, Vol. 33, No. 8, 08.1988, p. 931-937.

Research output: Contribution to journalArticle

Rubin, NH, Singh, P, Alinder, G, Greeley, GH, Rayford, PL, Rietveld, WOPJ & Thompson, JC 1988, 'Circadian rhythms in gastrin receptors in rat fundic stomach', Digestive Diseases and Sciences, vol. 33, no. 8, pp. 931-937. https://doi.org/10.1007/BF01535987
Rubin NH, Singh P, Alinder G, Greeley GH, Rayford PL, Rietveld WOPJ et al. Circadian rhythms in gastrin receptors in rat fundic stomach. Digestive Diseases and Sciences. 1988 Aug;33(8):931-937. https://doi.org/10.1007/BF01535987
Rubin, Norma H. ; Singh, Pomila ; Alinder, Gunnar ; Greeley, George H. ; Rayford, Phillip L. ; Rietveld, WOP J. ; Thompson, James C. / Circadian rhythms in gastrin receptors in rat fundic stomach. In: Digestive Diseases and Sciences. 1988 ; Vol. 33, No. 8. pp. 931-937.
@article{c37c2344d2864f94b3c74d532fbfea58,
title = "Circadian rhythms in gastrin receptors in rat fundic stomach",
abstract = "Circadian rhythmicity in the number of gastrin receptors in rat fundic mucosa was characterized and was related to the concentrations of gastrin in serum and in antrum. Male Sprague-Dawley rats were acclimated to 12 hr light alternating with 12 hr darkness. Subgroups of six rats each were killed at 4-hr intervals. Fundic mucosa was collected for measurement of gastrin receptors; serum and antral tissues were collected for measurement of gastrin levels by radioimmunoassay. Circadian periodicity in the data was determined by cosinor analyses. In both freely fed and fasted rats, gastrin receptors showed circadian variation (range 2.5-10 fmol/mg protein), as did serum gastrin concentrations (range in fed rats 195-407 pg/ml). The phasing of the intrinsic circadian variation in gastrin receptor level that was observed in the fasted rats was advanced by a few hours in fed rats. This shift is probably due to food-induced gastrin release, resulting in gastrin-mediated down-regulation of gastrin receptors, followed by up-regulation of gastrin receptors. Food-related effects were thus superimposed upon the intrinsic circadian rhythms in gastrin receptor levels, causing the circadian variation in gastrin receptor levels in the fed rats to be shifted forward compared to that in the fasted rats. No significant circadian rhythms, on the other other hand, were found in concentrations of gastrin in the antrum. These results suggest that changes in sensitivity of target tissues to hormones are related to both intrinsic circadian rhythms in levels of hormone receptors and also to food-related changes in hormone-receptor levels mediated by changing serum hormone levels.",
keywords = "circadian rhythms, gastrin, gastrin receptors, rat, stomach",
author = "Rubin, {Norma H.} and Pomila Singh and Gunnar Alinder and Greeley, {George H.} and Rayford, {Phillip L.} and Rietveld, {WOP J.} and Thompson, {James C.}",
year = "1988",
month = "8",
doi = "10.1007/BF01535987",
language = "English",
volume = "33",
pages = "931--937",
journal = "Digestive Diseases and Sciences",
issn = "0163-2116",
publisher = "Springer New York",
number = "8",

}

TY - JOUR

T1 - Circadian rhythms in gastrin receptors in rat fundic stomach

AU - Rubin, Norma H.

AU - Singh, Pomila

AU - Alinder, Gunnar

AU - Greeley, George H.

AU - Rayford, Phillip L.

AU - Rietveld, WOP J.

AU - Thompson, James C.

PY - 1988/8

Y1 - 1988/8

N2 - Circadian rhythmicity in the number of gastrin receptors in rat fundic mucosa was characterized and was related to the concentrations of gastrin in serum and in antrum. Male Sprague-Dawley rats were acclimated to 12 hr light alternating with 12 hr darkness. Subgroups of six rats each were killed at 4-hr intervals. Fundic mucosa was collected for measurement of gastrin receptors; serum and antral tissues were collected for measurement of gastrin levels by radioimmunoassay. Circadian periodicity in the data was determined by cosinor analyses. In both freely fed and fasted rats, gastrin receptors showed circadian variation (range 2.5-10 fmol/mg protein), as did serum gastrin concentrations (range in fed rats 195-407 pg/ml). The phasing of the intrinsic circadian variation in gastrin receptor level that was observed in the fasted rats was advanced by a few hours in fed rats. This shift is probably due to food-induced gastrin release, resulting in gastrin-mediated down-regulation of gastrin receptors, followed by up-regulation of gastrin receptors. Food-related effects were thus superimposed upon the intrinsic circadian rhythms in gastrin receptor levels, causing the circadian variation in gastrin receptor levels in the fed rats to be shifted forward compared to that in the fasted rats. No significant circadian rhythms, on the other other hand, were found in concentrations of gastrin in the antrum. These results suggest that changes in sensitivity of target tissues to hormones are related to both intrinsic circadian rhythms in levels of hormone receptors and also to food-related changes in hormone-receptor levels mediated by changing serum hormone levels.

AB - Circadian rhythmicity in the number of gastrin receptors in rat fundic mucosa was characterized and was related to the concentrations of gastrin in serum and in antrum. Male Sprague-Dawley rats were acclimated to 12 hr light alternating with 12 hr darkness. Subgroups of six rats each were killed at 4-hr intervals. Fundic mucosa was collected for measurement of gastrin receptors; serum and antral tissues were collected for measurement of gastrin levels by radioimmunoassay. Circadian periodicity in the data was determined by cosinor analyses. In both freely fed and fasted rats, gastrin receptors showed circadian variation (range 2.5-10 fmol/mg protein), as did serum gastrin concentrations (range in fed rats 195-407 pg/ml). The phasing of the intrinsic circadian variation in gastrin receptor level that was observed in the fasted rats was advanced by a few hours in fed rats. This shift is probably due to food-induced gastrin release, resulting in gastrin-mediated down-regulation of gastrin receptors, followed by up-regulation of gastrin receptors. Food-related effects were thus superimposed upon the intrinsic circadian rhythms in gastrin receptor levels, causing the circadian variation in gastrin receptor levels in the fed rats to be shifted forward compared to that in the fasted rats. No significant circadian rhythms, on the other other hand, were found in concentrations of gastrin in the antrum. These results suggest that changes in sensitivity of target tissues to hormones are related to both intrinsic circadian rhythms in levels of hormone receptors and also to food-related changes in hormone-receptor levels mediated by changing serum hormone levels.

KW - circadian rhythms

KW - gastrin

KW - gastrin receptors

KW - rat

KW - stomach

UR - http://www.scopus.com/inward/record.url?scp=0023721568&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023721568&partnerID=8YFLogxK

U2 - 10.1007/BF01535987

DO - 10.1007/BF01535987

M3 - Article

VL - 33

SP - 931

EP - 937

JO - Digestive Diseases and Sciences

JF - Digestive Diseases and Sciences

SN - 0163-2116

IS - 8

ER -