Circulating calcitonin gene-related peptide and its placental origins in normotensive and preeclamptic pregnancies

Yuan Lin Dong, Madhu Chauhan, Kortney E. Green, Sujatha Vegiraju, Hui Qun Wang, Gary D.V. Hankins, Chandra Yallampalli

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

Objective: The present study was designed to determine plasma calcitonin gene-related peptide concentration in both maternal and fetal circulations in normotensive and pre-eclamptic pregnancies and investigate whether placenta is 1 of its origins. Study design: Maternal blood, cord blood, and villous tissue were collected from women in normotensive pregnancies and complicated with pre-eclampsia. Calcitonin gene-related peptide concentrations were determined by radioimmunoassay. Cellular localizations of calcitonin gene-related peptide messenger ribonucleic acid and protein expressions in placental villi were determined by in situ hybridization and immunohistochemistry. Results: The following results were reached: (1) maternal plasma calcitonin gene-related peptide concentrations increased with advancing gestation but fell after delivery; (2) both maternal and cord plasma calcitonin gene-related peptide concentrations were positively correlated with the infant birth weights; (3) compared with normotensive pregnancies, calcitonin gene-related peptide levels in both maternal and cord plasma decreased in pregnancies with pre-eclampsia; (4) in normotensive pregnancies, the plasma calcitonin gene-related peptide of the umbilical vein was higher than the umbilical artery, but no significant differences between vein and artery in pre-eclampsia; (5) calcitonin gene-related peptide messenger ribonucleic acid and protein were expressed by syncytiotrophoblast cells and villous vascular endothelial cells in normotensive pregnancies, but only weak or absent staining was observed in pre-eclamptic placentas; and (6) calcitonin gene-related peptide is secreted by villous tissue in explant culture in a time-dependent manner, but less calcitonin gene-related peptide was produced by villous tissues from patients with pre-eclampsia. Conclusion: Calcitonin gene-related peptide may play potential roles in maternal hemodynamic adaptation and fetal growth. Decreased circulating calcitonin gene-related peptide levels may be involved in maternal-fetal pathophysiology of pre-eclampsia. It is novel that placenta villous tissues might be one of the potential sources of calcitonin gene-related peptide during pregnancy.

Original languageEnglish (US)
Pages (from-to)1657-1667
Number of pages11
JournalAmerican journal of obstetrics and gynecology
Volume195
Issue number6
DOIs
StatePublished - Dec 1 2006

Keywords

  • Calcitonin gene-related peptide
  • Placenta
  • Pre-eclampsia
  • Pregnancy

ASJC Scopus subject areas

  • Obstetrics and Gynecology

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    Dong, Y. L., Chauhan, M., Green, K. E., Vegiraju, S., Wang, H. Q., Hankins, G. D. V., & Yallampalli, C. (2006). Circulating calcitonin gene-related peptide and its placental origins in normotensive and preeclamptic pregnancies. American journal of obstetrics and gynecology, 195(6), 1657-1667. https://doi.org/10.1016/j.ajog.2006.04.006