Circulating miR-192 in liver fluke-associated cholangiocarcinoma patients: A prospective prognostic indicator

Runglawan Silakit, Watcharin Loilome, Puangrat Yongvanit, Porncheera Chusorn, Anchalee Techasen, Thidarut Boonmars, Narong Khuntikeo, Nittaya Chamadol, Chawalit Pairojkul, Nisana Namwat

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Background This study aimed to investigate the miR-192 levels in patients' sera of liver fluke-associated cholangiocarcinoma (CCA) for a prospective prognostic indicator.

Methods MicroRNA polymerase chain reaction (PCR) array was performed using pooled serum samples from 11 CCA patients and nine healthy subjects. Selected miRNAs were verified for the differential levels in both sera and tumor tissues (of patients and Opisthorchis viverrini (Ov)-induced CCA model) using TaqMan miRNA expression assay.

Results Our results demonstrated that miR-192 was significantly higher in the serum of CCA patients than that in healthy subjects giving a sensitivity of 74% and specificity of 72% (area under the curve [AUC] = 0.803; 95% confidence interval [CI], 0.708-0.897, P < 0.0001). Serum miR-192 examined in Ov infected subjects and subjects with periductal fibrosis were increased but not statistically significantly when compared with healthy subjects. High levels of serum miR-192 were significantly correlated with lymph node metastasis (P = 0.047) and shorter survival compared with individuals with low levels of serum miR-192 (hazard ratio [HR] 2.076, 95% CI 1.004-4.291, P = 0.049). We also found that the expression levels of miR-192 appeared to be elevated in both CCA tissues of patients and in Ov-induced CCA tissues of a hamster model.

Conclusions This finding indicates that elevated levels of miR-192 may be involved in CCA genesis and have a potential utility as a noninvasive prognostic indicator for CCA patients.

Original languageEnglish (US)
Pages (from-to)864-872
Number of pages9
JournalJournal of Hepato-Biliary-Pancreatic Sciences
Volume21
Issue number12
DOIs
StatePublished - Dec 1 2014
Externally publishedYes

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Fasciola hepatica
Cholangiocarcinoma
Opisthorchis
Serum
MicroRNAs
Healthy Volunteers
Confidence Intervals
Cricetinae
Area Under Curve
Fibrosis
Lymph Nodes
Neoplasm Metastasis
Sensitivity and Specificity
Polymerase Chain Reaction
Survival

Keywords

  • Cholangiocarcinoma
  • Opisthorchis viverrini
  • Prognostic indicator
  • Serum miR-192

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Circulating miR-192 in liver fluke-associated cholangiocarcinoma patients : A prospective prognostic indicator. / Silakit, Runglawan; Loilome, Watcharin; Yongvanit, Puangrat; Chusorn, Porncheera; Techasen, Anchalee; Boonmars, Thidarut; Khuntikeo, Narong; Chamadol, Nittaya; Pairojkul, Chawalit; Namwat, Nisana.

In: Journal of Hepato-Biliary-Pancreatic Sciences, Vol. 21, No. 12, 01.12.2014, p. 864-872.

Research output: Contribution to journalArticle

Silakit, R, Loilome, W, Yongvanit, P, Chusorn, P, Techasen, A, Boonmars, T, Khuntikeo, N, Chamadol, N, Pairojkul, C & Namwat, N 2014, 'Circulating miR-192 in liver fluke-associated cholangiocarcinoma patients: A prospective prognostic indicator', Journal of Hepato-Biliary-Pancreatic Sciences, vol. 21, no. 12, pp. 864-872. https://doi.org/10.1002/jhbp.145
Silakit, Runglawan ; Loilome, Watcharin ; Yongvanit, Puangrat ; Chusorn, Porncheera ; Techasen, Anchalee ; Boonmars, Thidarut ; Khuntikeo, Narong ; Chamadol, Nittaya ; Pairojkul, Chawalit ; Namwat, Nisana. / Circulating miR-192 in liver fluke-associated cholangiocarcinoma patients : A prospective prognostic indicator. In: Journal of Hepato-Biliary-Pancreatic Sciences. 2014 ; Vol. 21, No. 12. pp. 864-872.
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abstract = "Background This study aimed to investigate the miR-192 levels in patients' sera of liver fluke-associated cholangiocarcinoma (CCA) for a prospective prognostic indicator.Methods MicroRNA polymerase chain reaction (PCR) array was performed using pooled serum samples from 11 CCA patients and nine healthy subjects. Selected miRNAs were verified for the differential levels in both sera and tumor tissues (of patients and Opisthorchis viverrini (Ov)-induced CCA model) using TaqMan miRNA expression assay.Results Our results demonstrated that miR-192 was significantly higher in the serum of CCA patients than that in healthy subjects giving a sensitivity of 74{\%} and specificity of 72{\%} (area under the curve [AUC] = 0.803; 95{\%} confidence interval [CI], 0.708-0.897, P < 0.0001). Serum miR-192 examined in Ov infected subjects and subjects with periductal fibrosis were increased but not statistically significantly when compared with healthy subjects. High levels of serum miR-192 were significantly correlated with lymph node metastasis (P = 0.047) and shorter survival compared with individuals with low levels of serum miR-192 (hazard ratio [HR] 2.076, 95{\%} CI 1.004-4.291, P = 0.049). We also found that the expression levels of miR-192 appeared to be elevated in both CCA tissues of patients and in Ov-induced CCA tissues of a hamster model.Conclusions This finding indicates that elevated levels of miR-192 may be involved in CCA genesis and have a potential utility as a noninvasive prognostic indicator for CCA patients.",
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T1 - Circulating miR-192 in liver fluke-associated cholangiocarcinoma patients

T2 - A prospective prognostic indicator

AU - Silakit, Runglawan

AU - Loilome, Watcharin

AU - Yongvanit, Puangrat

AU - Chusorn, Porncheera

AU - Techasen, Anchalee

AU - Boonmars, Thidarut

AU - Khuntikeo, Narong

AU - Chamadol, Nittaya

AU - Pairojkul, Chawalit

AU - Namwat, Nisana

PY - 2014/12/1

Y1 - 2014/12/1

N2 - Background This study aimed to investigate the miR-192 levels in patients' sera of liver fluke-associated cholangiocarcinoma (CCA) for a prospective prognostic indicator.Methods MicroRNA polymerase chain reaction (PCR) array was performed using pooled serum samples from 11 CCA patients and nine healthy subjects. Selected miRNAs were verified for the differential levels in both sera and tumor tissues (of patients and Opisthorchis viverrini (Ov)-induced CCA model) using TaqMan miRNA expression assay.Results Our results demonstrated that miR-192 was significantly higher in the serum of CCA patients than that in healthy subjects giving a sensitivity of 74% and specificity of 72% (area under the curve [AUC] = 0.803; 95% confidence interval [CI], 0.708-0.897, P < 0.0001). Serum miR-192 examined in Ov infected subjects and subjects with periductal fibrosis were increased but not statistically significantly when compared with healthy subjects. High levels of serum miR-192 were significantly correlated with lymph node metastasis (P = 0.047) and shorter survival compared with individuals with low levels of serum miR-192 (hazard ratio [HR] 2.076, 95% CI 1.004-4.291, P = 0.049). We also found that the expression levels of miR-192 appeared to be elevated in both CCA tissues of patients and in Ov-induced CCA tissues of a hamster model.Conclusions This finding indicates that elevated levels of miR-192 may be involved in CCA genesis and have a potential utility as a noninvasive prognostic indicator for CCA patients.

AB - Background This study aimed to investigate the miR-192 levels in patients' sera of liver fluke-associated cholangiocarcinoma (CCA) for a prospective prognostic indicator.Methods MicroRNA polymerase chain reaction (PCR) array was performed using pooled serum samples from 11 CCA patients and nine healthy subjects. Selected miRNAs were verified for the differential levels in both sera and tumor tissues (of patients and Opisthorchis viverrini (Ov)-induced CCA model) using TaqMan miRNA expression assay.Results Our results demonstrated that miR-192 was significantly higher in the serum of CCA patients than that in healthy subjects giving a sensitivity of 74% and specificity of 72% (area under the curve [AUC] = 0.803; 95% confidence interval [CI], 0.708-0.897, P < 0.0001). Serum miR-192 examined in Ov infected subjects and subjects with periductal fibrosis were increased but not statistically significantly when compared with healthy subjects. High levels of serum miR-192 were significantly correlated with lymph node metastasis (P = 0.047) and shorter survival compared with individuals with low levels of serum miR-192 (hazard ratio [HR] 2.076, 95% CI 1.004-4.291, P = 0.049). We also found that the expression levels of miR-192 appeared to be elevated in both CCA tissues of patients and in Ov-induced CCA tissues of a hamster model.Conclusions This finding indicates that elevated levels of miR-192 may be involved in CCA genesis and have a potential utility as a noninvasive prognostic indicator for CCA patients.

KW - Cholangiocarcinoma

KW - Opisthorchis viverrini

KW - Prognostic indicator

KW - Serum miR-192

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