Circulating miRNA Expression Profiling and Target Prediction in Patients Receiving Dexmedetomidine

Xuehui Yang, Hongmei Chen, Yan Chen, Yochai Birnbaum, Rongbi Liang, Yumei Ye, Jinqiao Qian

Research output: Contribution to journalArticlepeer-review

16 Scopus citations


Background/Aims: Circulating miRNAs could serve as biomarkers for diagnosis or prognosis of heart diseases and cerebrovascular diseases. Dexmedetomidine has protective effects in various organs. The effects of dexmedetomidine on circulating miRNAs remain unknown. Here, we investigated differentially expressed miRNA and to predict the target genes of the miRNA in patients receiving dexmedetomidine. Methods: The expression levels of circulating miRNAs of 3 patients were determined through high through-put miRNA sequencing technology. Target genes of the identified differentially expressed miRNAs were predicted using TargetScan 7.1 and miRDB v.5. Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) were used to conduct functional annotation and pathway enrichment analysis of target genes respectively. Results: Twelve differentially expressed miRNAs were identified. Five miRNAs were upregulated (hsa-miR-4508, hsa-miR-novel-chr8_87373, hsa-miR-30a-3p, hsa-miR-novel-chr16_26099, hsa-miR-4306) and seven miRNAs (hsa-miR-744-5p, hsa-miR-320a, hsa-miR-novel-chr9_90035, hsa-miR-101-3p, hsa-miR-150-5p, hsa-miR-342-3p, and hsa-miR-140-3p) were downregulated after administration of dexmedetomidine in the subjects. The target genes and pathways related to the differentially expressed miRNAs were predicted and analyzed. Conclusion: The differentially expressed miRNAs may be involved in the mechanisms of action of dexmedetomidine. Specific miRNAs, such as hsa-miR-101-3p, hsa-miR-150-5p and hsa-miR-140-3p, are new potential targets for further functional studies of dexmedetomidine.

Original languageEnglish (US)
Pages (from-to)569-584
Number of pages16
JournalCellular Physiology and Biochemistry
Issue number2
StatePublished - Oct 1 2018
Externally publishedYes


  • Cardioprotection
  • Circulating miRNAs
  • Dexmedetomidine
  • Expression profling
  • Ischemia/reperfusion injury

ASJC Scopus subject areas

  • General Medicine


Dive into the research topics of 'Circulating miRNA Expression Profiling and Target Prediction in Patients Receiving Dexmedetomidine'. Together they form a unique fingerprint.

Cite this