Circulating miRNA profiles of doxorubicin-induced cardiotoxicity in breast cancer patients

Valentina K. Todorova, Issam Makhoul, Jeanne Wei, Vicki Klimberg

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Doxorubicin (DOX) cardiotoxicity is unpredictable and begins with the first dose of chemotherapy. This study aimed to obtain information about circulating microRNA of cancer patients in the early dose of DOX chemotherapy, who either did or did not develop cardiac abnormality after the completion of chemotherapy. Plasma of 20 patients treated for breast cancer with DOX-chemotherapy was analyzed using quantitative RT-PCR. Circulating microRNA profiles of patients with DOX cardiotoxicity were compared to microRNA profiles of patients without DOX cardiotoxicity by quantitative real-time PCR. Thirty-two microRNAs were significantly dysregulated in the patients with abnormal cardiac function. Functional analysis of the 32 miRNAs suggested association with cell death, cell cycle, and inflammation. We have identified a miRNA signature associated with early doses of DOX-based chemotherapy that may potentially predict later impairment of cardiac function in breast cancer patients. Our data lay a foundation for future studies to identify biomarkers for presymptomatic DOX-induced cardiotoxicity.

Original languageEnglish (US)
Pages (from-to)115-119
Number of pages5
JournalAnnals of Clinical and Laboratory Science
Volume47
Issue number2
StatePublished - Mar 1 2017
Externally publishedYes

Fingerprint

MicroRNAs
Doxorubicin
Chemotherapy
Breast Neoplasms
Drug Therapy
Functional analysis
Biomarkers
Cell death
Cardiotoxicity
Real-Time Polymerase Chain Reaction
Cell Cycle
Cell Death
Cells
Association reactions
Inflammation
Plasmas
Polymerase Chain Reaction
Neoplasms

ASJC Scopus subject areas

  • Immunology and Allergy
  • Microbiology
  • Pathology and Forensic Medicine
  • Immunology
  • Hematology
  • Molecular Biology
  • Clinical Biochemistry
  • Medical Laboratory Technology

Cite this

Circulating miRNA profiles of doxorubicin-induced cardiotoxicity in breast cancer patients. / Todorova, Valentina K.; Makhoul, Issam; Wei, Jeanne; Klimberg, Vicki.

In: Annals of Clinical and Laboratory Science, Vol. 47, No. 2, 01.03.2017, p. 115-119.

Research output: Contribution to journalArticle

Todorova, Valentina K. ; Makhoul, Issam ; Wei, Jeanne ; Klimberg, Vicki. / Circulating miRNA profiles of doxorubicin-induced cardiotoxicity in breast cancer patients. In: Annals of Clinical and Laboratory Science. 2017 ; Vol. 47, No. 2. pp. 115-119.
@article{671f3705975a47ad8c54d7ba16ae0df0,
title = "Circulating miRNA profiles of doxorubicin-induced cardiotoxicity in breast cancer patients",
abstract = "Doxorubicin (DOX) cardiotoxicity is unpredictable and begins with the first dose of chemotherapy. This study aimed to obtain information about circulating microRNA of cancer patients in the early dose of DOX chemotherapy, who either did or did not develop cardiac abnormality after the completion of chemotherapy. Plasma of 20 patients treated for breast cancer with DOX-chemotherapy was analyzed using quantitative RT-PCR. Circulating microRNA profiles of patients with DOX cardiotoxicity were compared to microRNA profiles of patients without DOX cardiotoxicity by quantitative real-time PCR. Thirty-two microRNAs were significantly dysregulated in the patients with abnormal cardiac function. Functional analysis of the 32 miRNAs suggested association with cell death, cell cycle, and inflammation. We have identified a miRNA signature associated with early doses of DOX-based chemotherapy that may potentially predict later impairment of cardiac function in breast cancer patients. Our data lay a foundation for future studies to identify biomarkers for presymptomatic DOX-induced cardiotoxicity.",
author = "Todorova, {Valentina K.} and Issam Makhoul and Jeanne Wei and Vicki Klimberg",
year = "2017",
month = "3",
day = "1",
language = "English (US)",
volume = "47",
pages = "115--119",
journal = "Annals of Clinical and Laboratory Science",
issn = "0091-7370",
publisher = "Association of Clinical Scientists",
number = "2",

}

TY - JOUR

T1 - Circulating miRNA profiles of doxorubicin-induced cardiotoxicity in breast cancer patients

AU - Todorova, Valentina K.

AU - Makhoul, Issam

AU - Wei, Jeanne

AU - Klimberg, Vicki

PY - 2017/3/1

Y1 - 2017/3/1

N2 - Doxorubicin (DOX) cardiotoxicity is unpredictable and begins with the first dose of chemotherapy. This study aimed to obtain information about circulating microRNA of cancer patients in the early dose of DOX chemotherapy, who either did or did not develop cardiac abnormality after the completion of chemotherapy. Plasma of 20 patients treated for breast cancer with DOX-chemotherapy was analyzed using quantitative RT-PCR. Circulating microRNA profiles of patients with DOX cardiotoxicity were compared to microRNA profiles of patients without DOX cardiotoxicity by quantitative real-time PCR. Thirty-two microRNAs were significantly dysregulated in the patients with abnormal cardiac function. Functional analysis of the 32 miRNAs suggested association with cell death, cell cycle, and inflammation. We have identified a miRNA signature associated with early doses of DOX-based chemotherapy that may potentially predict later impairment of cardiac function in breast cancer patients. Our data lay a foundation for future studies to identify biomarkers for presymptomatic DOX-induced cardiotoxicity.

AB - Doxorubicin (DOX) cardiotoxicity is unpredictable and begins with the first dose of chemotherapy. This study aimed to obtain information about circulating microRNA of cancer patients in the early dose of DOX chemotherapy, who either did or did not develop cardiac abnormality after the completion of chemotherapy. Plasma of 20 patients treated for breast cancer with DOX-chemotherapy was analyzed using quantitative RT-PCR. Circulating microRNA profiles of patients with DOX cardiotoxicity were compared to microRNA profiles of patients without DOX cardiotoxicity by quantitative real-time PCR. Thirty-two microRNAs were significantly dysregulated in the patients with abnormal cardiac function. Functional analysis of the 32 miRNAs suggested association with cell death, cell cycle, and inflammation. We have identified a miRNA signature associated with early doses of DOX-based chemotherapy that may potentially predict later impairment of cardiac function in breast cancer patients. Our data lay a foundation for future studies to identify biomarkers for presymptomatic DOX-induced cardiotoxicity.

UR - http://www.scopus.com/inward/record.url?scp=85018448045&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85018448045&partnerID=8YFLogxK

M3 - Article

C2 - 28442511

AN - SCOPUS:85018448045

VL - 47

SP - 115

EP - 119

JO - Annals of Clinical and Laboratory Science

JF - Annals of Clinical and Laboratory Science

SN - 0091-7370

IS - 2

ER -