CK2α regulates the transcription of BRP in Drosophila

Yogesh Wairkar, Deepti Trivedi, Rajalaxmi Natarajan, Kevin Barnes, Lhia Dolores, Phillip Cho

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Development and plasticity of synapses are brought about by a complex interplay between various signaling pathways. Typically, either changing the number of synapses or strengthening an existing synapse can lead to changes during synaptic plasticity. Altering the machinery that governs the exocytosis of synaptic vesicles, which primarily fuse at specialized structures known as active zones on the presynaptic terminal, brings about these changes. Although signaling pathways that regulate the synaptic plasticity from the postsynaptic compartments are well defined, the pathways that control these changes presynaptically are poorly described. In a genetic screen for synapse development in Drosophila, we found that mutations in CK2α lead to an increase in the levels of Bruchpilot (BRP), a scaffolding protein associated with the active zones. Using a combination of genetic and biochemical approaches, we found that the increase in BRP in CK2α mutants is largely due to an increase in the transcription of BRP. Interestingly, the transcripts of other active zone proteins that are important for function of active zones were also increased, while the transcripts from some other synaptic proteins were unchanged. Thus, our data suggest that CK2α might be important in regulating synaptic plasticity by modulating the transcription of BRP. Hence, we propose that CK2α is a novel regulator of the active zone protein, BRP, in Drosophila.

Original languageEnglish (US)
Pages (from-to)53-64
Number of pages12
JournalDevelopmental Biology
Volume384
Issue number1
DOIs
StatePublished - Dec 1 2013

Fingerprint

Synapses
Drosophila
Neuronal Plasticity
Proteins
Synaptic Vesicles
Exocytosis
Presynaptic Terminals
Molecular Biology
Mutation

Keywords

  • Active zone
  • Bruchpilot
  • Drosophila
  • Neuromuscular junction
  • Synapse
  • Synapse development

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology
  • Molecular Biology

Cite this

Wairkar, Y., Trivedi, D., Natarajan, R., Barnes, K., Dolores, L., & Cho, P. (2013). CK2α regulates the transcription of BRP in Drosophila. Developmental Biology, 384(1), 53-64. https://doi.org/10.1016/j.ydbio.2013.09.025

CK2α regulates the transcription of BRP in Drosophila. / Wairkar, Yogesh; Trivedi, Deepti; Natarajan, Rajalaxmi; Barnes, Kevin; Dolores, Lhia; Cho, Phillip.

In: Developmental Biology, Vol. 384, No. 1, 01.12.2013, p. 53-64.

Research output: Contribution to journalArticle

Wairkar, Y, Trivedi, D, Natarajan, R, Barnes, K, Dolores, L & Cho, P 2013, 'CK2α regulates the transcription of BRP in Drosophila', Developmental Biology, vol. 384, no. 1, pp. 53-64. https://doi.org/10.1016/j.ydbio.2013.09.025
Wairkar, Yogesh ; Trivedi, Deepti ; Natarajan, Rajalaxmi ; Barnes, Kevin ; Dolores, Lhia ; Cho, Phillip. / CK2α regulates the transcription of BRP in Drosophila. In: Developmental Biology. 2013 ; Vol. 384, No. 1. pp. 53-64.
@article{4f107fd268744c3da6fed5c6bc5a4c8a,
title = "CK2α regulates the transcription of BRP in Drosophila",
abstract = "Development and plasticity of synapses are brought about by a complex interplay between various signaling pathways. Typically, either changing the number of synapses or strengthening an existing synapse can lead to changes during synaptic plasticity. Altering the machinery that governs the exocytosis of synaptic vesicles, which primarily fuse at specialized structures known as active zones on the presynaptic terminal, brings about these changes. Although signaling pathways that regulate the synaptic plasticity from the postsynaptic compartments are well defined, the pathways that control these changes presynaptically are poorly described. In a genetic screen for synapse development in Drosophila, we found that mutations in CK2α lead to an increase in the levels of Bruchpilot (BRP), a scaffolding protein associated with the active zones. Using a combination of genetic and biochemical approaches, we found that the increase in BRP in CK2α mutants is largely due to an increase in the transcription of BRP. Interestingly, the transcripts of other active zone proteins that are important for function of active zones were also increased, while the transcripts from some other synaptic proteins were unchanged. Thus, our data suggest that CK2α might be important in regulating synaptic plasticity by modulating the transcription of BRP. Hence, we propose that CK2α is a novel regulator of the active zone protein, BRP, in Drosophila.",
keywords = "Active zone, Bruchpilot, Drosophila, Neuromuscular junction, Synapse, Synapse development",
author = "Yogesh Wairkar and Deepti Trivedi and Rajalaxmi Natarajan and Kevin Barnes and Lhia Dolores and Phillip Cho",
year = "2013",
month = "12",
day = "1",
doi = "10.1016/j.ydbio.2013.09.025",
language = "English (US)",
volume = "384",
pages = "53--64",
journal = "Developmental Biology",
issn = "0012-1606",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - CK2α regulates the transcription of BRP in Drosophila

AU - Wairkar, Yogesh

AU - Trivedi, Deepti

AU - Natarajan, Rajalaxmi

AU - Barnes, Kevin

AU - Dolores, Lhia

AU - Cho, Phillip

PY - 2013/12/1

Y1 - 2013/12/1

N2 - Development and plasticity of synapses are brought about by a complex interplay between various signaling pathways. Typically, either changing the number of synapses or strengthening an existing synapse can lead to changes during synaptic plasticity. Altering the machinery that governs the exocytosis of synaptic vesicles, which primarily fuse at specialized structures known as active zones on the presynaptic terminal, brings about these changes. Although signaling pathways that regulate the synaptic plasticity from the postsynaptic compartments are well defined, the pathways that control these changes presynaptically are poorly described. In a genetic screen for synapse development in Drosophila, we found that mutations in CK2α lead to an increase in the levels of Bruchpilot (BRP), a scaffolding protein associated with the active zones. Using a combination of genetic and biochemical approaches, we found that the increase in BRP in CK2α mutants is largely due to an increase in the transcription of BRP. Interestingly, the transcripts of other active zone proteins that are important for function of active zones were also increased, while the transcripts from some other synaptic proteins were unchanged. Thus, our data suggest that CK2α might be important in regulating synaptic plasticity by modulating the transcription of BRP. Hence, we propose that CK2α is a novel regulator of the active zone protein, BRP, in Drosophila.

AB - Development and plasticity of synapses are brought about by a complex interplay between various signaling pathways. Typically, either changing the number of synapses or strengthening an existing synapse can lead to changes during synaptic plasticity. Altering the machinery that governs the exocytosis of synaptic vesicles, which primarily fuse at specialized structures known as active zones on the presynaptic terminal, brings about these changes. Although signaling pathways that regulate the synaptic plasticity from the postsynaptic compartments are well defined, the pathways that control these changes presynaptically are poorly described. In a genetic screen for synapse development in Drosophila, we found that mutations in CK2α lead to an increase in the levels of Bruchpilot (BRP), a scaffolding protein associated with the active zones. Using a combination of genetic and biochemical approaches, we found that the increase in BRP in CK2α mutants is largely due to an increase in the transcription of BRP. Interestingly, the transcripts of other active zone proteins that are important for function of active zones were also increased, while the transcripts from some other synaptic proteins were unchanged. Thus, our data suggest that CK2α might be important in regulating synaptic plasticity by modulating the transcription of BRP. Hence, we propose that CK2α is a novel regulator of the active zone protein, BRP, in Drosophila.

KW - Active zone

KW - Bruchpilot

KW - Drosophila

KW - Neuromuscular junction

KW - Synapse

KW - Synapse development

UR - http://www.scopus.com/inward/record.url?scp=84886951459&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84886951459&partnerID=8YFLogxK

U2 - 10.1016/j.ydbio.2013.09.025

DO - 10.1016/j.ydbio.2013.09.025

M3 - Article

VL - 384

SP - 53

EP - 64

JO - Developmental Biology

JF - Developmental Biology

SN - 0012-1606

IS - 1

ER -