Abstract
Food poisoning due to staphylococcal enterotoxins (SEs) affects hundreds of thousands of people each year. Little is known about how SEs initiate immune responses and cause pathogenesis. Here, we demonstrate that cultured human intestinal myofibroblasts (IMFs) bind SEs in an MHC class II-dependent fashion. IMFs respond to SE exposure with increased secretion of IL-6, IL-8, and TNF-α. A significant proliferative T cell response was observed when MHC class II-expressing IMFs were pulsed with SEA and cocultured with human CD4 + T cells. In conclusion, our findings support the hypothesis that IMFs may play an important role in pathology associated with staphlococcocal enterotoxigenic disease.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 313-318 |
| Number of pages | 6 |
| Journal | Annals of the New York Academy of Sciences |
| Volume | 1029 |
| DOIs | |
| State | Published - 2004 |
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Keywords
- Cytokines
- MHC class II molecule
- Myofibroblast
- Staphylococcal enterotoxins
- Superantigen
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
Cite this
Class II MHC-expressing myofibroblasts play a role in the immunopathogenesis associated with staphylococcal enterotoxins. / Barrera, C. A.; Pinchuk, Iryna; Saada, J. I.; Suarez, G.; Bland, D. A.; Beswick, E.; Adegboyega, P. A.; Mifflin, R. C.; Powell, D. W.; Reyes, Victor.
In: Annals of the New York Academy of Sciences, Vol. 1029, 2004, p. 313-318.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Class II MHC-expressing myofibroblasts play a role in the immunopathogenesis associated with staphylococcal enterotoxins
AU - Barrera, C. A.
AU - Pinchuk, Iryna
AU - Saada, J. I.
AU - Suarez, G.
AU - Bland, D. A.
AU - Beswick, E.
AU - Adegboyega, P. A.
AU - Mifflin, R. C.
AU - Powell, D. W.
AU - Reyes, Victor
PY - 2004
Y1 - 2004
N2 - Food poisoning due to staphylococcal enterotoxins (SEs) affects hundreds of thousands of people each year. Little is known about how SEs initiate immune responses and cause pathogenesis. Here, we demonstrate that cultured human intestinal myofibroblasts (IMFs) bind SEs in an MHC class II-dependent fashion. IMFs respond to SE exposure with increased secretion of IL-6, IL-8, and TNF-α. A significant proliferative T cell response was observed when MHC class II-expressing IMFs were pulsed with SEA and cocultured with human CD4 + T cells. In conclusion, our findings support the hypothesis that IMFs may play an important role in pathology associated with staphlococcocal enterotoxigenic disease.
AB - Food poisoning due to staphylococcal enterotoxins (SEs) affects hundreds of thousands of people each year. Little is known about how SEs initiate immune responses and cause pathogenesis. Here, we demonstrate that cultured human intestinal myofibroblasts (IMFs) bind SEs in an MHC class II-dependent fashion. IMFs respond to SE exposure with increased secretion of IL-6, IL-8, and TNF-α. A significant proliferative T cell response was observed when MHC class II-expressing IMFs were pulsed with SEA and cocultured with human CD4 + T cells. In conclusion, our findings support the hypothesis that IMFs may play an important role in pathology associated with staphlococcocal enterotoxigenic disease.
KW - Cytokines
KW - MHC class II molecule
KW - Myofibroblast
KW - Staphylococcal enterotoxins
KW - Superantigen
UR - http://www.scopus.com/inward/record.url?scp=20044367101&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=20044367101&partnerID=8YFLogxK
U2 - 10.1196/annals.1309.022
DO - 10.1196/annals.1309.022
M3 - Article
VL - 1029
SP - 313
EP - 318
JO - Annals of the New York Academy of Sciences
JF - Annals of the New York Academy of Sciences
SN - 0077-8923
ER -