Cleavage of Poly(A)-Binding Protein by Poliovirus 3C Protease Inhibits Host Cell Translation: A Novel Mechanism for Host Translation Shutoff

Neslihan Martinez, Marc E. Van Eden, Patrick Younan, Richard E. Lloyd

Research output: Contribution to journalArticle

137 Citations (Scopus)

Abstract

Cleavage of eukaryotic translation initiation factor 4GI (eIF4GI) by viral 2A protease (2Apro) has been proposed to cause severe translation inhibition in poliovirus-infected cells. However, infections containing 1 mM guanidine-HCI result in eIF4GI cleavage but only partial translation shutoff, indicating eIF4GI cleavage is insufficient for drastic translation inhibition. Viral 3C protease (3Cpro) cleaves poly(A)-binding protein (PABP) and removes the C-terminal domain (CTD) that interacts with several translation factors. In HeLa cell translation extracts that exhibit cap-poly(A) synergy, partial cleavage of PABP by 3Cpro inhibited translation of endogenous mRNAs and reporter RNA as effectively as complete cleavage of eIF4GI and eIF4GII by 2Apro. 3Cpro-mediated translation inhibition was poly(A) dependent, and addition of PABP to extracts restored translation. Expression of 3Cpro in HeLa cells resulted in partial PABP cleavage and similar inhibition of translation. PABP cleavage did not affect eIF4GI-PABP interactions, and the results of kinetics experiments suggest that 3Cpro might inhibit late steps in translation or ribosome recycling. The data illustrate the importance of the CTD of PABP in poly(A)-dependent translation in mammalian cells. We propose that enteroviruses use a dual strategy for host translation shutoff, requiring cleavage of PABP by 3Cpro and of eIF4G by 2Apro.

Original languageEnglish (US)
Pages (from-to)1779-1790
Number of pages12
JournalMolecular and Cellular Biology
Volume24
Issue number4
DOIs
StatePublished - Feb 2004
Externally publishedYes

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Poly(A)-Binding Proteins
Eukaryotic Initiation Factors
Poly A
Peptide Hydrolases
HeLa Cells
Poliovirus
3C proteases
Enterovirus
Guanidine
Protein Biosynthesis
Protein C
Cell Extracts
Ribosomes
RNA

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Cleavage of Poly(A)-Binding Protein by Poliovirus 3C Protease Inhibits Host Cell Translation : A Novel Mechanism for Host Translation Shutoff. / Martinez, Neslihan; Van Eden, Marc E.; Younan, Patrick; Lloyd, Richard E.

In: Molecular and Cellular Biology, Vol. 24, No. 4, 02.2004, p. 1779-1790.

Research output: Contribution to journalArticle

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