Clinical and histological effects of the intrathecal administration of a single dose of dexmedetomidine in rabbits

Hugo D. de Pereira Cardoso, Natalia C. Fim, Mariangela A. Marques, Hélio Miot, Vânia M. de Vasconcelos Machado, Daneshvari R. Solanki, Rodrigo Moreira Lima, Ana L. de Carvalho, Lais H. Navarro, Eliana Marisa Ganem

    Research output: Contribution to journalArticle

    2 Citations (Scopus)

    Abstract

    Background: There is experimental evidence that dexmedetomidine has neuroprotective effects. So, it could be expected that its intrathecal or epidural administration presents no harm. However, whether dexmedetomidine is neurotoxic to the spinal cord remains to be fully elucidated. Objective: To evaluate the effect of preservative-free dexmedetomidine administered as a subarachnoid single injection on the spinal cord and meninges of rabbits. Study Design: Research article. Setting: Experimental research laboratory. Methods: Twenty young adult female rabbits, each weighing between 3200 and 4900 g, and having a spine length between 36 and 40 cm, were divided by lot into 2 groups (G): 0.9% saline in G1 and preservative-free dexmedetomidine in G2 (dose of 10 μg). After intravenous anesthesia with ketamine and xylazine, the subarachnoid space was punctured at S1-S2 under ultrasound guidance, and a random 5 μl.cm-1 of spinal length (0.2 mL) of solution (saline or dexmedetomidine) was injected. The animals remained in captivity for 21 days under medical observation and were sacrificed by decapitation. The lumbosacral spinal cord portion was removed for immunohistochemistry to assess the glial fibrillary acidic protein (GFAP), and histology was assessed using hematoxylin and eosin (HE) stain. Results: None of the animals had impaired motor function or decreased nociception during the period of clinical observation. None of the animals from the control group showed signs of injuries to meninges. In the dexmedetomidine group, however, 9 animals presented with signs of meningeal injury. The main histological changes observed were areas with meningeal thickening and lymphoplasmocitary infiltration in the pia-mater and arachnoid. Further histological examination also revealed adherence areas among the pia and arachnoid. There was no signal of injury in neural tissue in any animal of both groups. Limitations: Evaluation of the possible analgesic effects of the intrathecal dexmedetomidine was not performed. Conclusion: On the basis of the present results, dexmedetomidine administered in the subarachnoid space in a single dose of 10 μg is capable of producing histological changes over the meninges of rabbits.

    Original languageEnglish (US)
    Pages (from-to)E319-E327
    JournalPain Physician
    Volume19
    Issue number2
    StatePublished - Feb 1 2016

    Fingerprint

    Dexmedetomidine
    Rabbits
    Meninges
    Arachnoid
    Spinal Cord
    Subarachnoid Space
    Wounds and Injuries
    Pia Mater
    Observation
    Xylazine
    Decapitation
    Intravenous Anesthesia
    Nociception
    Glial Fibrillary Acidic Protein
    Ketamine
    Neuroprotective Agents
    Hematoxylin
    Eosine Yellowish-(YS)
    Sodium Chloride
    Analgesics

    Keywords

    • Anesthesia, spinal
    • Dexmedetomine
    • Injections, spinal
    • Rabbits
    • Spinal cord

    ASJC Scopus subject areas

    • Anesthesiology and Pain Medicine

    Cite this

    de Pereira Cardoso, H. D., Fim, N. C., Marques, M. A., Miot, H., de Vasconcelos Machado, V. M., Solanki, D. R., ... Ganem, E. M. (2016). Clinical and histological effects of the intrathecal administration of a single dose of dexmedetomidine in rabbits. Pain Physician, 19(2), E319-E327.

    Clinical and histological effects of the intrathecal administration of a single dose of dexmedetomidine in rabbits. / de Pereira Cardoso, Hugo D.; Fim, Natalia C.; Marques, Mariangela A.; Miot, Hélio; de Vasconcelos Machado, Vânia M.; Solanki, Daneshvari R.; Lima, Rodrigo Moreira; de Carvalho, Ana L.; Navarro, Lais H.; Ganem, Eliana Marisa.

    In: Pain Physician, Vol. 19, No. 2, 01.02.2016, p. E319-E327.

    Research output: Contribution to journalArticle

    de Pereira Cardoso, HD, Fim, NC, Marques, MA, Miot, H, de Vasconcelos Machado, VM, Solanki, DR, Lima, RM, de Carvalho, AL, Navarro, LH & Ganem, EM 2016, 'Clinical and histological effects of the intrathecal administration of a single dose of dexmedetomidine in rabbits', Pain Physician, vol. 19, no. 2, pp. E319-E327.
    de Pereira Cardoso HD, Fim NC, Marques MA, Miot H, de Vasconcelos Machado VM, Solanki DR et al. Clinical and histological effects of the intrathecal administration of a single dose of dexmedetomidine in rabbits. Pain Physician. 2016 Feb 1;19(2):E319-E327.
    de Pereira Cardoso, Hugo D. ; Fim, Natalia C. ; Marques, Mariangela A. ; Miot, Hélio ; de Vasconcelos Machado, Vânia M. ; Solanki, Daneshvari R. ; Lima, Rodrigo Moreira ; de Carvalho, Ana L. ; Navarro, Lais H. ; Ganem, Eliana Marisa. / Clinical and histological effects of the intrathecal administration of a single dose of dexmedetomidine in rabbits. In: Pain Physician. 2016 ; Vol. 19, No. 2. pp. E319-E327.
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    AU - de Pereira Cardoso, Hugo D.

    AU - Fim, Natalia C.

    AU - Marques, Mariangela A.

    AU - Miot, Hélio

    AU - de Vasconcelos Machado, Vânia M.

    AU - Solanki, Daneshvari R.

    AU - Lima, Rodrigo Moreira

    AU - de Carvalho, Ana L.

    AU - Navarro, Lais H.

    AU - Ganem, Eliana Marisa

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    N2 - Background: There is experimental evidence that dexmedetomidine has neuroprotective effects. So, it could be expected that its intrathecal or epidural administration presents no harm. However, whether dexmedetomidine is neurotoxic to the spinal cord remains to be fully elucidated. Objective: To evaluate the effect of preservative-free dexmedetomidine administered as a subarachnoid single injection on the spinal cord and meninges of rabbits. Study Design: Research article. Setting: Experimental research laboratory. Methods: Twenty young adult female rabbits, each weighing between 3200 and 4900 g, and having a spine length between 36 and 40 cm, were divided by lot into 2 groups (G): 0.9% saline in G1 and preservative-free dexmedetomidine in G2 (dose of 10 μg). After intravenous anesthesia with ketamine and xylazine, the subarachnoid space was punctured at S1-S2 under ultrasound guidance, and a random 5 μl.cm-1 of spinal length (0.2 mL) of solution (saline or dexmedetomidine) was injected. The animals remained in captivity for 21 days under medical observation and were sacrificed by decapitation. The lumbosacral spinal cord portion was removed for immunohistochemistry to assess the glial fibrillary acidic protein (GFAP), and histology was assessed using hematoxylin and eosin (HE) stain. Results: None of the animals had impaired motor function or decreased nociception during the period of clinical observation. None of the animals from the control group showed signs of injuries to meninges. In the dexmedetomidine group, however, 9 animals presented with signs of meningeal injury. The main histological changes observed were areas with meningeal thickening and lymphoplasmocitary infiltration in the pia-mater and arachnoid. Further histological examination also revealed adherence areas among the pia and arachnoid. There was no signal of injury in neural tissue in any animal of both groups. Limitations: Evaluation of the possible analgesic effects of the intrathecal dexmedetomidine was not performed. Conclusion: On the basis of the present results, dexmedetomidine administered in the subarachnoid space in a single dose of 10 μg is capable of producing histological changes over the meninges of rabbits.

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    KW - Dexmedetomine

    KW - Injections, spinal

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