Clinical, hematologic, and humoral immune response in female dogs inoculated with Rickettsia rickettsii and Rickettsia montana.

E. B. Breitschwerdt, David Walker, M. G. Levy, W. Burgdorfer, W. T. Corbett, S. A. Hurlbert, M. E. Stebbins, B. C. Curtis, D. A. Allen

Research output: Contribution to journalArticle

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Abstract

Female Beagles were inoculated intradermally with a sublethal dose of Rickettsia rickettsii and R montana. Three dogs (group 1) were inoculated with 2 X 10(2) plaque-forming units (PFU) of R rickettsia and were treated with tetracycline beginning on postinoculation day (PID) 12; 3 dogs (group 2) were inoculated with 2 X 10(2) PFU of R rickettsii but were not treated; 3 dogs (group 3) were inoculated with 2 X 10(2) PFU of R montana. Group-3 dogs failed to seroconvert and were inoculated a second time on PID 68. Groups 1 and 2 dogs inoculated with R rickettsii became depressed and developed occasional inappetence, fever, hematochezia, and ocular lesions. These dogs had a decrease in PCV and RBC count, an initial decrease in WBC count followed by leukocytosis, and a decrease in platelet count. Group-3 dogs inoculated with R montana remained healthy. After R rickettsii inoculation, the serologic response to spotted fever group (SFG) rickettsial antigens (R rickettsii, R rhipicephali, R montana, and R bellii) was similar. The antibody response to R rickettsii was first detected on PID 9, with peak titers reached by PID 20. Serum titers to R rickettsii remained stable or decreased one dilution through PID 120. Of 4 SFG rickettsial antigens, the highest serologic response was to R rickettsii. A cross-reacting antibody response with R rhipicephali and R montana was nearly identical and was only slightly less than the response to R rickettsii. Cross-reacting antibodies to R belli were of lower mean titer and of shorter duration than were cross-reacting antibodies to other SFG rickettsiae.(ABSTRACT TRUNCATED AT 250 WORDS)

Original languageEnglish (US)
Pages (from-to)70-76
Number of pages7
JournalAmerican Journal of Veterinary Research
Volume49
Issue number1
StatePublished - Jan 1988
Externally publishedYes

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Rickettsia montanensis
Rickettsia rickettsii
Rickettsia
Humoral Immunity
humoral immunity
Dogs
dogs
fever
Fever
antibodies
Antibody Formation
Bellis
antigens
Antigens
Gastrointestinal Hemorrhage
blood platelet count
Antibodies
Leukocytosis
erythrocyte count
Tetracycline

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Breitschwerdt, E. B., Walker, D., Levy, M. G., Burgdorfer, W., Corbett, W. T., Hurlbert, S. A., ... Allen, D. A. (1988). Clinical, hematologic, and humoral immune response in female dogs inoculated with Rickettsia rickettsii and Rickettsia montana. American Journal of Veterinary Research, 49(1), 70-76.

Clinical, hematologic, and humoral immune response in female dogs inoculated with Rickettsia rickettsii and Rickettsia montana. / Breitschwerdt, E. B.; Walker, David; Levy, M. G.; Burgdorfer, W.; Corbett, W. T.; Hurlbert, S. A.; Stebbins, M. E.; Curtis, B. C.; Allen, D. A.

In: American Journal of Veterinary Research, Vol. 49, No. 1, 01.1988, p. 70-76.

Research output: Contribution to journalArticle

Breitschwerdt, EB, Walker, D, Levy, MG, Burgdorfer, W, Corbett, WT, Hurlbert, SA, Stebbins, ME, Curtis, BC & Allen, DA 1988, 'Clinical, hematologic, and humoral immune response in female dogs inoculated with Rickettsia rickettsii and Rickettsia montana.', American Journal of Veterinary Research, vol. 49, no. 1, pp. 70-76.
Breitschwerdt, E. B. ; Walker, David ; Levy, M. G. ; Burgdorfer, W. ; Corbett, W. T. ; Hurlbert, S. A. ; Stebbins, M. E. ; Curtis, B. C. ; Allen, D. A. / Clinical, hematologic, and humoral immune response in female dogs inoculated with Rickettsia rickettsii and Rickettsia montana. In: American Journal of Veterinary Research. 1988 ; Vol. 49, No. 1. pp. 70-76.
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abstract = "Female Beagles were inoculated intradermally with a sublethal dose of Rickettsia rickettsii and R montana. Three dogs (group 1) were inoculated with 2 X 10(2) plaque-forming units (PFU) of R rickettsia and were treated with tetracycline beginning on postinoculation day (PID) 12; 3 dogs (group 2) were inoculated with 2 X 10(2) PFU of R rickettsii but were not treated; 3 dogs (group 3) were inoculated with 2 X 10(2) PFU of R montana. Group-3 dogs failed to seroconvert and were inoculated a second time on PID 68. Groups 1 and 2 dogs inoculated with R rickettsii became depressed and developed occasional inappetence, fever, hematochezia, and ocular lesions. These dogs had a decrease in PCV and RBC count, an initial decrease in WBC count followed by leukocytosis, and a decrease in platelet count. Group-3 dogs inoculated with R montana remained healthy. After R rickettsii inoculation, the serologic response to spotted fever group (SFG) rickettsial antigens (R rickettsii, R rhipicephali, R montana, and R bellii) was similar. The antibody response to R rickettsii was first detected on PID 9, with peak titers reached by PID 20. Serum titers to R rickettsii remained stable or decreased one dilution through PID 120. Of 4 SFG rickettsial antigens, the highest serologic response was to R rickettsii. A cross-reacting antibody response with R rhipicephali and R montana was nearly identical and was only slightly less than the response to R rickettsii. Cross-reacting antibodies to R belli were of lower mean titer and of shorter duration than were cross-reacting antibodies to other SFG rickettsiae.(ABSTRACT TRUNCATED AT 250 WORDS)",
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