Abstract
We studied the usefulness of repeated enzyme-linked immunosorbent assay testing for heparin-induced thrombocytopenia (HIT) when the initial test result was negative and sought to determine whether the titer of the initial negative result correlated with the likelihood of obtaining a positive test result in repeated testing. We divided 150 patients who underwent HIT testing into 3 groups (50 patients each): (1) very low titer negative (0.0%-33.3% of the threshold for a positive test); (2) low titer negative (33.4%-66.6% of the threshold); and (3) high titer negative (66.7%-99.9% of the threshold). Among the patients who underwent a repeat test, 5% (1/20) of group 1 patients, 13% (4/32) of group 2 patients, and 43% (13/30) of group 3 patients tested positive in the repeat test (P = .0026). Thus, nearly half of patients with initially negative HIT test results had positive results in the repeat test if the negative titer was 66.7% or more of the threshold. If laboratories report the HIT titer, rather than just negative or positive, the titer might help clinicians predict which patients have HIT despite a negative initial test, and the overall sensitivity for diagnosing HIT might be improved.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 61-65 |
| Number of pages | 5 |
| Journal | American Journal of Clinical Pathology |
| Volume | 119 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 1 2003 |
| Externally published | Yes |
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Keywords
- ELISA
- Heparin
- Heparin-induced thrombocytopenia
- HIT titer
- Platelet count
- Thrombocytopenia
- Thrombosis
ASJC Scopus subject areas
- Pathology and Forensic Medicine
Cite this
Clinical significance of a borderline titer in a negative ELISA test for heparin-induced thrombocytopenia. / Refaai, Majed A.; Laposata, Michael; Van Cott, Elizabeth M.
In: American Journal of Clinical Pathology, Vol. 119, No. 1, 01.01.2003, p. 61-65.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Clinical significance of a borderline titer in a negative ELISA test for heparin-induced thrombocytopenia
AU - Refaai, Majed A.
AU - Laposata, Michael
AU - Van Cott, Elizabeth M.
PY - 2003/1/1
Y1 - 2003/1/1
N2 - We studied the usefulness of repeated enzyme-linked immunosorbent assay testing for heparin-induced thrombocytopenia (HIT) when the initial test result was negative and sought to determine whether the titer of the initial negative result correlated with the likelihood of obtaining a positive test result in repeated testing. We divided 150 patients who underwent HIT testing into 3 groups (50 patients each): (1) very low titer negative (0.0%-33.3% of the threshold for a positive test); (2) low titer negative (33.4%-66.6% of the threshold); and (3) high titer negative (66.7%-99.9% of the threshold). Among the patients who underwent a repeat test, 5% (1/20) of group 1 patients, 13% (4/32) of group 2 patients, and 43% (13/30) of group 3 patients tested positive in the repeat test (P = .0026). Thus, nearly half of patients with initially negative HIT test results had positive results in the repeat test if the negative titer was 66.7% or more of the threshold. If laboratories report the HIT titer, rather than just negative or positive, the titer might help clinicians predict which patients have HIT despite a negative initial test, and the overall sensitivity for diagnosing HIT might be improved.
AB - We studied the usefulness of repeated enzyme-linked immunosorbent assay testing for heparin-induced thrombocytopenia (HIT) when the initial test result was negative and sought to determine whether the titer of the initial negative result correlated with the likelihood of obtaining a positive test result in repeated testing. We divided 150 patients who underwent HIT testing into 3 groups (50 patients each): (1) very low titer negative (0.0%-33.3% of the threshold for a positive test); (2) low titer negative (33.4%-66.6% of the threshold); and (3) high titer negative (66.7%-99.9% of the threshold). Among the patients who underwent a repeat test, 5% (1/20) of group 1 patients, 13% (4/32) of group 2 patients, and 43% (13/30) of group 3 patients tested positive in the repeat test (P = .0026). Thus, nearly half of patients with initially negative HIT test results had positive results in the repeat test if the negative titer was 66.7% or more of the threshold. If laboratories report the HIT titer, rather than just negative or positive, the titer might help clinicians predict which patients have HIT despite a negative initial test, and the overall sensitivity for diagnosing HIT might be improved.
KW - ELISA
KW - Heparin
KW - Heparin-induced thrombocytopenia
KW - HIT titer
KW - Platelet count
KW - Thrombocytopenia
KW - Thrombosis
UR - http://www.scopus.com/inward/record.url?scp=85047688615&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85047688615&partnerID=8YFLogxK
U2 - 10.1309/6922EWGPHVDX9EQJ
DO - 10.1309/6922EWGPHVDX9EQJ
M3 - Article
C2 - 12520698
AN - SCOPUS:85047688615
VL - 119
SP - 61
EP - 65
JO - American Journal of Clinical Pathology
JF - American Journal of Clinical Pathology
SN - 0002-9173
IS - 1
ER -