Clinical validation of an RIA for natural and recombinant erythropoietin in serun and plasma

Mark A. Goldberg, Thomas J. Schneider, Shaista Khan, John R. Petersen

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

A sensitive radioimmunoassay (RIA) for the detection of erythropoietin (EPO) was developed using antibody directed against EPO from human urine. With 100 μL of sample, the assay is sensitive to 7 U/L, well below the mean EPO level in normal males (15.1 ± 3.5 U/L) or females (15.4 ± 4.8 U/L). Dilutions of a variety of human serum samples show a parallel relationship with the standard EPO. Clinical validation of the RIA was confirmed by appropriate increases or decreases of EPO levels in various types of anemia and polycythemia. Serum EPO levels were also measured in volunteers participating in an autologous blood donation study. The RIA proved to eb quite sensitve, detecting small increases even after a single unit phlebotomy. This RIA of human EPO meets all the requirements of a routine clinical assay in terms of specificity and clinical sensitivity and can be easily conducted in routine clinical laboratories.

Original languageEnglish (US)
Pages (from-to)183-189
Number of pages7
JournalClinical Biochemistry
Volume26
Issue number3
DOIs
StatePublished - Jun 1993

Keywords

  • anemia
  • erythrocytosis
  • hematologic malignancy
  • phlebotomy
  • polycythemia
  • renal failure

ASJC Scopus subject areas

  • Clinical Biochemistry

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