Clinical, virologic, and immunologic follow-up of convalescent Ebola hemorrhagic fever patients and their household contacts, Kikwit, Democratic Republic of the Congo

Alexander K. Rowe, Jeanne Bertolli, Ali S. Khan, Rose Mukunu, J. J. Muyembe-Tamfum, David Bressler, A. J. Williams, C. J. Peters, Luis Rodriguez, Heinz Feldmann, Stuart T. Nichol, Pierre E. Rollin, Thomas Ksiazek

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Abstract

A cohort of convalescent Ebola hemorrhagic fever (EHF) patients and their household contacts (HHCs) were studied prospectively to determine if convalescent body fluids contain Ebola virus and if secondary transmission occurs during convalescence. Twenty-nine EHF convalescents and 152 HHCs were monitored for up to 21 months. Blood specimens were obtained and symptom information was collected from convalescents and their HHCs; other body fluid specimens were also obtained from convalescents. Arthralgias and myalgia were reported significantly more often by convalescents than HHCs. Evidence of Ebola virus was detected by reverse transcription-polymerase chain reaction in semen specimens up to 91 days after disease onset; however, these and all other non-blood body fluids tested negative by virus isolation. Among 81 initially antibody negative HHCs, none became antibody positive. Blood specimens of 5 HHCs not identified as EHF patients were initially antibody positive. No direct evidence of convalescent-to-HHC transmission of EHF was found, although the semen of convalescents may be infectious. The existence of initially antibody-positive HHCs suggests that mild cases of Ebola virus infection occurred and that the full extent of the EHF epidemic was probably underestimated.

Original languageEnglish (US)
JournalJournal of Infectious Diseases
Volume179
Issue numberSUPPL. 1
StatePublished - 1999
Externally publishedYes

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Ebola Hemorrhagic Fever
Democratic Republic of the Congo
Body Fluids
Ebolavirus
Antibodies
Semen
Myalgia
Arthralgia
Reverse Transcription
Viruses

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Immunology

Cite this

Clinical, virologic, and immunologic follow-up of convalescent Ebola hemorrhagic fever patients and their household contacts, Kikwit, Democratic Republic of the Congo. / Rowe, Alexander K.; Bertolli, Jeanne; Khan, Ali S.; Mukunu, Rose; Muyembe-Tamfum, J. J.; Bressler, David; Williams, A. J.; Peters, C. J.; Rodriguez, Luis; Feldmann, Heinz; Nichol, Stuart T.; Rollin, Pierre E.; Ksiazek, Thomas.

In: Journal of Infectious Diseases, Vol. 179, No. SUPPL. 1, 1999.

Research output: Contribution to journalArticle

Rowe, AK, Bertolli, J, Khan, AS, Mukunu, R, Muyembe-Tamfum, JJ, Bressler, D, Williams, AJ, Peters, CJ, Rodriguez, L, Feldmann, H, Nichol, ST, Rollin, PE & Ksiazek, T 1999, 'Clinical, virologic, and immunologic follow-up of convalescent Ebola hemorrhagic fever patients and their household contacts, Kikwit, Democratic Republic of the Congo', Journal of Infectious Diseases, vol. 179, no. SUPPL. 1.
Rowe, Alexander K. ; Bertolli, Jeanne ; Khan, Ali S. ; Mukunu, Rose ; Muyembe-Tamfum, J. J. ; Bressler, David ; Williams, A. J. ; Peters, C. J. ; Rodriguez, Luis ; Feldmann, Heinz ; Nichol, Stuart T. ; Rollin, Pierre E. ; Ksiazek, Thomas. / Clinical, virologic, and immunologic follow-up of convalescent Ebola hemorrhagic fever patients and their household contacts, Kikwit, Democratic Republic of the Congo. In: Journal of Infectious Diseases. 1999 ; Vol. 179, No. SUPPL. 1.
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