Cloning and bioinformatic analysis of PqERF1 gene in Panax quinquefolius

Yong Zhen Sun, Yun Yun Niu, Ying Li, Yingjie Zhu, Hong Mei Luo, Shi Lin Chen

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

ERF family transcription factor (TF) represented ethylene-responsive protein which harbored a conserved AP2 domain. After searching the plant transcription factor database, a total of 75 unigenes was found which contained AP2 domain from the transcriptome dataset of Panax quinquefolius L. One unique sequence of ERF transcript, named as PqERF1, was cloned with entire open reading frame of 933 base pairs (bp). Protein prediction result indicated that the gene was localized in nucleus and had a conserved AP2 domain. PqERF1 gene could be induced by methyl jasmonate (MeJA) which was consistent to the inducing profile of triterpene ginsenosides. InterproScan prediction indicated that PqERF1 was probably a pathogenesis-related gene. Sequence alignment and phylogenetic analysis demonstrated PqERF1 was with high identity and had relative close relationship to the NtERF4 (Nicotiana tabacum), PhERF12 (Petunia x hybrida) and DcERF1 (Daucus carota) which was related to plant defense, regulation of secondary metabolism and the flower senescence respectively. Therefore, the gene was likely involved in regulation of secondary metabolism, plant defense and physical processes which would provide gene resource for further study on secondary metabolite synthesis and molecular breeding of P. quinquefolius.

Original languageEnglish (US)
Pages (from-to)1008-1014
Number of pages7
JournalYaoxue Xuebao
Volume46
Issue number8
StatePublished - Aug 12 2011
Externally publishedYes

Keywords

  • Ethylene response factor
  • Panax quinquefolius
  • Plant defense
  • Real-time PCR
  • Secondary metabolism

ASJC Scopus subject areas

  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Molecular Medicine

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    Sun, Y. Z., Niu, Y. Y., Li, Y., Zhu, Y., Luo, H. M., & Chen, S. L. (2011). Cloning and bioinformatic analysis of PqERF1 gene in Panax quinquefolius. Yaoxue Xuebao, 46(8), 1008-1014.