Cloning and characterization of a histone gene family in Tilapia fish

Ella Englander, Boaz Moav

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Genomic organization and expression of a histone gene family in the Tilapia (Cichlidae) fish was determined. A genomic library was prepared; four clones containing the complete set of core histones were analyzed. These clones differed significantly in their restriction maps, although three of them revealed a uniform internai gene arrangement of the core histone genes. Differential expression between two of the cloned clusters was observed when the hybridization pattern of those clusters to various RNA preparations was compared. The copy number of histone genes was estimated to be 100 to 110 for haploid Tilapia genome.

Original languageEnglish (US)
Pages (from-to)277-282
Number of pages6
JournalBBA - Gene Structure and Expression
Volume1007
Issue number3
DOIs
StatePublished - Apr 12 1989
Externally publishedYes

Fingerprint

Tilapia
Cloning
Histones
Fish
Organism Cloning
Fishes
Genes
Clone Cells
Cichlids
Gene Order
Gene Dosage
Genomic Library
Haploidy
Genome
RNA

Keywords

  • (Tilapia fish)
  • Gene characterization
  • Gene cloning
  • Genomic organization
  • Histone gene family

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Genetics
  • Structural Biology
  • Medicine(all)

Cite this

Cloning and characterization of a histone gene family in Tilapia fish. / Englander, Ella; Moav, Boaz.

In: BBA - Gene Structure and Expression, Vol. 1007, No. 3, 12.04.1989, p. 277-282.

Research output: Contribution to journalArticle

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