Cloning and characterization of the Drosophila melanogaster CDK5 homolog

Mark R. Hellmich, James A. Kennison, Lori L. Hampton, James F. Battey

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

The D. melanogaster homolog of mammalian CDK5 has been cloned and its chromosomal location determined. The gene for Cdk5 consists of 4 exons separated by 3 short introns ranging in size from 61-160 bp. Northern blot analysis revealed a single mRNA of approximately 1.6 kb that is expressed at highest levels in the adult fly. The putative amino acid sequence for Drosophila Cdk5 predicts a protein with a mass of approximately 32 kDa that is 77% identical to its mammalian counter-parts. Drosophila Cdk5 gene is located in polytene chromosomal region 52BC of the right arm of chromosome 2. This study provides the framework for a molecular genetic analysis of CDK5 function.

Original languageEnglish (US)
Pages (from-to)317-321
Number of pages5
JournalFEBS Letters
Volume356
Issue number2-3
DOIs
StatePublished - Dec 19 1994
Externally publishedYes

Keywords

  • CDK5
  • Cyclin-dependent kinase
  • Development
  • Drosophila
  • cDNA

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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