Cloning and characterization of the Drosophila melanogaster CDK5 homolog

Mark R. Hellmich; James A. Kennison; Lori L. Hampton; James F. Battey

doi:10.1016/0014-5793(94)01298-9

Cloning and characterization of the Drosophila melanogaster CDK5 homolog

Mark R. Hellmich, James A. Kennison, Lori L. Hampton, James F. Battey

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

The D. melanogaster homolog of mammalian CDK5 has been cloned and its chromosomal location determined. The gene for Cdk5 consists of 4 exons separated by 3 short introns ranging in size from 61-160 bp. Northern blot analysis revealed a single mRNA of approximately 1.6 kb that is expressed at highest levels in the adult fly. The putative amino acid sequence for Drosophila Cdk5 predicts a protein with a mass of approximately 32 kDa that is 77% identical to its mammalian counter-parts. Drosophila Cdk5 gene is located in polytene chromosomal region 52BC of the right arm of chromosome 2. This study provides the framework for a molecular genetic analysis of CDK5 function.

Original language	English (US)
Pages (from-to)	317-321
Number of pages	5
Journal	FEBS Letters
Volume	356
Issue number	2-3
DOIs	https://doi.org/10.1016/0014-5793(94)01298-9
State	Published - Dec 19 1994
Externally published	Yes

Keywords

CDK5
Cyclin-dependent kinase
Development
Drosophila
cDNA

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/0014-5793(94)01298-9

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title = "Cloning and characterization of the Drosophila melanogaster CDK5 homolog",

abstract = "The D. melanogaster homolog of mammalian CDK5 has been cloned and its chromosomal location determined. The gene for Cdk5 consists of 4 exons separated by 3 short introns ranging in size from 61-160 bp. Northern blot analysis revealed a single mRNA of approximately 1.6 kb that is expressed at highest levels in the adult fly. The putative amino acid sequence for Drosophila Cdk5 predicts a protein with a mass of approximately 32 kDa that is 77% identical to its mammalian counter-parts. Drosophila Cdk5 gene is located in polytene chromosomal region 52BC of the right arm of chromosome 2. This study provides the framework for a molecular genetic analysis of CDK5 function.",

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year = "1994",

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T1 - Cloning and characterization of the Drosophila melanogaster CDK5 homolog

AU - Hellmich, Mark R.

AU - Kennison, James A.

AU - Hampton, Lori L.

AU - Battey, James F.

PY - 1994/12/19

Y1 - 1994/12/19

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AB - The D. melanogaster homolog of mammalian CDK5 has been cloned and its chromosomal location determined. The gene for Cdk5 consists of 4 exons separated by 3 short introns ranging in size from 61-160 bp. Northern blot analysis revealed a single mRNA of approximately 1.6 kb that is expressed at highest levels in the adult fly. The putative amino acid sequence for Drosophila Cdk5 predicts a protein with a mass of approximately 32 kDa that is 77% identical to its mammalian counter-parts. Drosophila Cdk5 gene is located in polytene chromosomal region 52BC of the right arm of chromosome 2. This study provides the framework for a molecular genetic analysis of CDK5 function.

KW - CDK5

KW - Cyclin-dependent kinase

KW - Development

KW - Drosophila

KW - cDNA

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Cloning and characterization of the Drosophila melanogaster CDK5 homolog

Abstract

Keywords

ASJC Scopus subject areas

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