Cloning and expression in Escherichia coli of the 37-, 14-, and/or 16-kilodalton antigens genes from Rickettsia prowazekii strain E.

L. P. Aniskovich, Vladimir Motin, N. M. Balayeva, G. B. Smirnov

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

A gene bank of Rickettsia prowazekii strain E constructed in the phage vector lambda EMBL4 was screened for antigen production with anti-R. prowazekii serum. One of the immunoreactive clones, grown at 37 degrees C exhibited the expression of at least two antigens of molecular weight (M(r)) 37 kD and 14 kD. Subcloning and further analysis revealed that the antigens (polypeptides) of Mr 37, 14, and/or 16 kD apparently represent structural units of the 138 kD complex antigen. Assembly of the above mentioned polypeptides was found to be thermosensitive as it took place at 30 degrees C but not at 37 degrees C and resulted in an oligomeric structure of M(r) 138 kD. The nucleotide sequence of the gene coding for a precursor of the mature polypeptides of Mr 14 and/or 16 kD was determined.

Original languageEnglish (US)
Pages (from-to)90-102
Number of pages13
JournalActa Virologica
Volume36
Issue number1
StatePublished - Jan 1992
Externally publishedYes

Fingerprint

Rickettsia prowazekii
Organism Cloning
Escherichia coli
Antigens
Peptides
Genes
Bacteriophage lambda
Clone Cells
Molecular Weight
Serum

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Cloning and expression in Escherichia coli of the 37-, 14-, and/or 16-kilodalton antigens genes from Rickettsia prowazekii strain E. / Aniskovich, L. P.; Motin, Vladimir; Balayeva, N. M.; Smirnov, G. B.

In: Acta Virologica, Vol. 36, No. 1, 01.1992, p. 90-102.

Research output: Contribution to journalArticle

@article{a58fdaf5505746da8f2c355546c4b65c,
title = "Cloning and expression in Escherichia coli of the 37-, 14-, and/or 16-kilodalton antigens genes from Rickettsia prowazekii strain E.",
abstract = "A gene bank of Rickettsia prowazekii strain E constructed in the phage vector lambda EMBL4 was screened for antigen production with anti-R. prowazekii serum. One of the immunoreactive clones, grown at 37 degrees C exhibited the expression of at least two antigens of molecular weight (M(r)) 37 kD and 14 kD. Subcloning and further analysis revealed that the antigens (polypeptides) of Mr 37, 14, and/or 16 kD apparently represent structural units of the 138 kD complex antigen. Assembly of the above mentioned polypeptides was found to be thermosensitive as it took place at 30 degrees C but not at 37 degrees C and resulted in an oligomeric structure of M(r) 138 kD. The nucleotide sequence of the gene coding for a precursor of the mature polypeptides of Mr 14 and/or 16 kD was determined.",
author = "Aniskovich, {L. P.} and Vladimir Motin and Balayeva, {N. M.} and Smirnov, {G. B.}",
year = "1992",
month = "1",
language = "English (US)",
volume = "36",
pages = "90--102",
journal = "Acta Virologica",
issn = "0001-723X",
publisher = "AEP - Academic Electronic Press Ltd.",
number = "1",

}

TY - JOUR

T1 - Cloning and expression in Escherichia coli of the 37-, 14-, and/or 16-kilodalton antigens genes from Rickettsia prowazekii strain E.

AU - Aniskovich, L. P.

AU - Motin, Vladimir

AU - Balayeva, N. M.

AU - Smirnov, G. B.

PY - 1992/1

Y1 - 1992/1

N2 - A gene bank of Rickettsia prowazekii strain E constructed in the phage vector lambda EMBL4 was screened for antigen production with anti-R. prowazekii serum. One of the immunoreactive clones, grown at 37 degrees C exhibited the expression of at least two antigens of molecular weight (M(r)) 37 kD and 14 kD. Subcloning and further analysis revealed that the antigens (polypeptides) of Mr 37, 14, and/or 16 kD apparently represent structural units of the 138 kD complex antigen. Assembly of the above mentioned polypeptides was found to be thermosensitive as it took place at 30 degrees C but not at 37 degrees C and resulted in an oligomeric structure of M(r) 138 kD. The nucleotide sequence of the gene coding for a precursor of the mature polypeptides of Mr 14 and/or 16 kD was determined.

AB - A gene bank of Rickettsia prowazekii strain E constructed in the phage vector lambda EMBL4 was screened for antigen production with anti-R. prowazekii serum. One of the immunoreactive clones, grown at 37 degrees C exhibited the expression of at least two antigens of molecular weight (M(r)) 37 kD and 14 kD. Subcloning and further analysis revealed that the antigens (polypeptides) of Mr 37, 14, and/or 16 kD apparently represent structural units of the 138 kD complex antigen. Assembly of the above mentioned polypeptides was found to be thermosensitive as it took place at 30 degrees C but not at 37 degrees C and resulted in an oligomeric structure of M(r) 138 kD. The nucleotide sequence of the gene coding for a precursor of the mature polypeptides of Mr 14 and/or 16 kD was determined.

UR - http://www.scopus.com/inward/record.url?scp=0026450579&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026450579&partnerID=8YFLogxK

M3 - Article

VL - 36

SP - 90

EP - 102

JO - Acta Virologica

JF - Acta Virologica

SN - 0001-723X

IS - 1

ER -