Cloning and expression of Buthus martensii Karsch scorpion toxin gene (BmK IT3) in Escherichia coli

Ji Bin Yu, Ping Ji, Xin Min Zha, Wei De Shen, Xiang Fu Wu

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4 Scopus citations

Abstract

According to the reported sequence of Buthus martensii Karsch scorpion toxin gene (BmK IT3), we synthesized two primers, which were complementary in a region. By the means of PCR, we got the gene. The gene was fused in expression vector pET-28a, which gave rise to a recombinant plasmid pET(IT3R). Then it was transformed into E. coli BL21 (DE3). With IPTG induction, the gene was efficiently expressed. And the fusion product was soluble.

Original languageEnglish (US)
Pages (from-to)106-108
Number of pages3
JournalSheng wu gong cheng xue bao = Chinese journal of biotechnology
Volume18
Issue number1
StatePublished - Jan 2002
Externally publishedYes

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ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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