According to the reported sequence of Buthus martensii Karsch scorpion toxin gene (BmK IT3), we synthesized two primers, which were complementary in a region. By the means of PCR, we got the gene. The gene was fused in expression vector pET-28a, which gave rise to a recombinant plasmid pET(IT3R). Then it was transformed into E. coli BL21 (DE3). With IPTG induction, the gene was efficiently expressed. And the fusion product was soluble.
|Original language||English (US)|
|Number of pages||3|
|Journal||Sheng wu gong cheng xue bao = Chinese journal of biotechnology|
|State||Published - Jan 2002|
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology