Cloning and expression of Buthus martensii Karsch scorpion toxin gene (BmK IT3) in Escherichia coli

Ji Bin Yu, Ping Ji, Xin Min Zha, Wei De Shen, Xiang Fu Wu

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

According to the reported sequence of Buthus martensii Karsch scorpion toxin gene (BmK IT3), we synthesized two primers, which were complementary in a region. By the means of PCR, we got the gene. The gene was fused in expression vector pET-28a, which gave rise to a recombinant plasmid pET(IT3R). Then it was transformed into E. coli BL21 (DE3). With IPTG induction, the gene was efficiently expressed. And the fusion product was soluble.

Original languageEnglish (US)
Pages (from-to)106-108
Number of pages3
JournalShengwu Gongcheng Xuebao/Chinese Journal of Biotechnology
Volume18
Issue number1
StatePublished - 2002
Externally publishedYes

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Scorpions
Organism Cloning
Escherichia coli
Genes
Isopropyl Thiogalactoside
Plasmids
Polymerase Chain Reaction
BmK IT(3) scorpion toxin

Cite this

Cloning and expression of Buthus martensii Karsch scorpion toxin gene (BmK IT3) in Escherichia coli. / Yu, Ji Bin; Ji, Ping; Zha, Xin Min; Shen, Wei De; Wu, Xiang Fu.

In: Shengwu Gongcheng Xuebao/Chinese Journal of Biotechnology, Vol. 18, No. 1, 2002, p. 106-108.

Research output: Contribution to journalArticle

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