Cloning and expression of Buthus martensii Karsch scorpion toxin gene (BmK IT3) in Escherichia coli

Ji Bin Yu; Ping Ji; Xin Min Zha; Wei De Shen; Xiang Fu Wu

Cloning and expression of Buthus martensii Karsch scorpion toxin gene (BmK IT3) in Escherichia coli

Ji Bin Yu
, Ping Ji
, Xin Min Zha
, Wei De Shen
, Xiang Fu Wu

Research output: Contribution to journal › Article › peer-review

Abstract

According to the reported sequence of Buthus martensii Karsch scorpion toxin gene (BmK IT3), we synthesized two primers, which were complementary in a region. By the means of PCR, we got the gene. The gene was fused in expression vector pET-28a, which gave rise to a recombinant plasmid pET(IT3R). Then it was transformed into E. coli BL21 (DE3). With IPTG induction, the gene was efficiently expressed. And the fusion product was soluble.

Original language	English (US)
Pages (from-to)	106-108
Number of pages	3
Journal	Sheng wu gong cheng xue bao = Chinese journal of biotechnology
Volume	18
Issue number	1
State	Published - Jan 2002
Externally published	Yes

ASJC Scopus subject areas

Biotechnology
Applied Microbiology and Biotechnology

Cite this

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title = "Cloning and expression of Buthus martensii Karsch scorpion toxin gene (BmK IT3) in Escherichia coli",

abstract = "According to the reported sequence of Buthus martensii Karsch scorpion toxin gene (BmK IT3), we synthesized two primers, which were complementary in a region. By the means of PCR, we got the gene. The gene was fused in expression vector pET-28a, which gave rise to a recombinant plasmid pET(IT3R). Then it was transformed into E. coli BL21 (DE3). With IPTG induction, the gene was efficiently expressed. And the fusion product was soluble.",

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year = "2002",

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journal = "Sheng wu gong cheng xue bao = Chinese journal of biotechnology",

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T1 - Cloning and expression of Buthus martensii Karsch scorpion toxin gene (BmK IT3) in Escherichia coli

AU - Yu, Ji Bin

AU - Ji, Ping

AU - Zha, Xin Min

AU - Shen, Wei De

AU - Wu, Xiang Fu

PY - 2002/1

Y1 - 2002/1

N2 - According to the reported sequence of Buthus martensii Karsch scorpion toxin gene (BmK IT3), we synthesized two primers, which were complementary in a region. By the means of PCR, we got the gene. The gene was fused in expression vector pET-28a, which gave rise to a recombinant plasmid pET(IT3R). Then it was transformed into E. coli BL21 (DE3). With IPTG induction, the gene was efficiently expressed. And the fusion product was soluble.

AB - According to the reported sequence of Buthus martensii Karsch scorpion toxin gene (BmK IT3), we synthesized two primers, which were complementary in a region. By the means of PCR, we got the gene. The gene was fused in expression vector pET-28a, which gave rise to a recombinant plasmid pET(IT3R). Then it was transformed into E. coli BL21 (DE3). With IPTG induction, the gene was efficiently expressed. And the fusion product was soluble.

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UR - https://www.scopus.com/pages/publications/0036365693#tab=citedBy

M3 - Article

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SN - 1000-3061

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JO - Sheng wu gong cheng xue bao = Chinese journal of biotechnology

JF - Sheng wu gong cheng xue bao = Chinese journal of biotechnology

IS - 1

ER -

Cloning and expression of Buthus martensii Karsch scorpion toxin gene (BmK IT3) in Escherichia coli

Abstract

ASJC Scopus subject areas

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