Cloning and expression of rat homeo-box-containing sequences

Miriam Falzon, Nancy Sanderson, Su Yun Chung

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Six rat homeo-box-containing DNA sequences have been isolated by screening a genomic library with a probe derived from an Antennapedia (Antp) cDNA clone of Drosophila melanogaster. Sequence determination of two of the clones containing the homeo-box regions reveals a 180-nucleotide(nt) domain sharing more than 80 % homology at the nucleotide level and more than 90 % homology at the amino acid level with the homeo-box from the Antp gene and from homeo-boxes of other metazoan species. Genomic blotting experiments suggest that the two homeo-box-containing DNA regions are present in one or two copies per haploid rat genome. Northern blot analysis of RNA has shown that the rat homeo-box sequences are expressed in a tissue-specific manner; transcripts were detected in the spinal cord and kidney, but not in brain, testis, liver, and spleen. The rat nucleotide sequences lying outside the 180-nt homeo-box domain share virtually no sequence homology with the Antp flanking regions. However, one clone does show an equally high degree of amino acid homology within the homeo-box and its immediate flanking region with a putative homologous gene in mouse. The result suggests that some of the mammalian homeo-box-containing genes are conserved in evolution and may serve important cellular or developmental functions.

Original languageEnglish (US)
Pages (from-to)23-32
Number of pages10
JournalGene
Volume54
Issue number1
DOIs
StatePublished - 1987
Externally publishedYes

Fingerprint

Homeobox Genes
Organism Cloning
Nucleotides
Clone Cells
Amino Acids
Genomic Library
Haploidy
Sequence Homology
Drosophila melanogaster
Northern Blotting
Sequence Analysis
Testis
Spinal Cord
Spleen
Complementary DNA
Genome
RNA
Kidney
Liver
DNA

Keywords

  • amino acid sequence conservation
  • Antp. gene
  • Drosophila melanogaster, Antennapedia
  • gene cloning
  • molecular evolution
  • phage λ Charon vectors
  • poly(A)RNA
  • Recombinant DNA

ASJC Scopus subject areas

  • Genetics

Cite this

Cloning and expression of rat homeo-box-containing sequences. / Falzon, Miriam; Sanderson, Nancy; Chung, Su Yun.

In: Gene, Vol. 54, No. 1, 1987, p. 23-32.

Research output: Contribution to journalArticle

Falzon, Miriam ; Sanderson, Nancy ; Chung, Su Yun. / Cloning and expression of rat homeo-box-containing sequences. In: Gene. 1987 ; Vol. 54, No. 1. pp. 23-32.
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