Cloning and nucleotide sequence analysis of the Saccharomyces cerevisiae RAD4 gene required for excision repair of UV-damaged DNA

R. Daniel Gietz, Satya Prakash

Research output: Contribution to journalArticle

27 Scopus citations

Abstract

The RAD4 gene of Saccharomyces cerevisiae is required for the incision step of excision repair. We have cloned the RAD4 gene and determined its nucleotide sequence. RAD4 encodes a somewhat basic protein of 754 amino acids (aa) with an Mr of 87173. RAD4 contains several groups of 4-7 consecutive basic aa residues that could be involved in DNA binding and it also contains an α-helix-turn-α-helix motif for DNA binding. Like several other DNA repair proteins of S. cerevisiae, the C terminus of RAD4 protein is highly acidic.

Original languageEnglish (US)
Pages (from-to)535-541
Number of pages7
JournalGene
Volume74
Issue number2
DOIs
StatePublished - Dec 30 1988
Externally publishedYes

Keywords

  • DNA binding
  • E. coli
  • Recombinant DNA
  • gap repair
  • histone binding
  • incision
  • toxic

ASJC Scopus subject areas

  • Genetics

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