Cloning and nucleotide sequence analysis of the Saccharomyces cerevisiae RAD4 gene required for excision repair of UV-damaged DNA

R. Daniel Gietz; Satya Prakash

doi:10.1016/0378-1119(88)90186-2

Cloning and nucleotide sequence analysis of the Saccharomyces cerevisiae RAD4 gene required for excision repair of UV-damaged DNA

R. Daniel Gietz
, Satya Prakash

Research output: Contribution to journal › Article › peer-review

31 Scopus citations

Abstract

The RAD4 gene of Saccharomyces cerevisiae is required for the incision step of excision repair. We have cloned the RAD4 gene and determined its nucleotide sequence. RAD4 encodes a somewhat basic protein of 754 amino acids (aa) with an M_r of 87173. RAD4 contains several groups of 4-7 consecutive basic aa residues that could be involved in DNA binding and it also contains an α-helix-turn-α-helix motif for DNA binding. Like several other DNA repair proteins of S. cerevisiae, the C terminus of RAD4 protein is highly acidic.

Original language	English (US)
Pages (from-to)	535-541
Number of pages	7
Journal	Gene
Volume	74
Issue number	2
DOIs	https://doi.org/10.1016/0378-1119(88)90186-2
State	Published - Dec 30 1988
Externally published	Yes

Keywords

DNA binding
E. coli
Recombinant DNA
gap repair
histone binding
incision
toxic

ASJC Scopus subject areas

Genetics

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10.1016/0378-1119(88)90186-2

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title = "Cloning and nucleotide sequence analysis of the Saccharomyces cerevisiae RAD4 gene required for excision repair of UV-damaged DNA",

abstract = "The RAD4 gene of Saccharomyces cerevisiae is required for the incision step of excision repair. We have cloned the RAD4 gene and determined its nucleotide sequence. RAD4 encodes a somewhat basic protein of 754 amino acids (aa) with an Mr of 87173. RAD4 contains several groups of 4-7 consecutive basic aa residues that could be involved in DNA binding and it also contains an α-helix-turn-α-helix motif for DNA binding. Like several other DNA repair proteins of S. cerevisiae, the C terminus of RAD4 protein is highly acidic.",

keywords = "DNA binding, E. coli, Recombinant DNA, gap repair, histone binding, incision, toxic",

author = "Gietz, \{R. Daniel\} and Satya Prakash",

year = "1988",

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doi = "10.1016/0378-1119(88)90186-2",

language = "English (US)",

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pages = "535--541",

journal = "Gene",

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TY - JOUR

T1 - Cloning and nucleotide sequence analysis of the Saccharomyces cerevisiae RAD4 gene required for excision repair of UV-damaged DNA

AU - Gietz, R. Daniel

AU - Prakash, Satya

PY - 1988/12/30

Y1 - 1988/12/30

N2 - The RAD4 gene of Saccharomyces cerevisiae is required for the incision step of excision repair. We have cloned the RAD4 gene and determined its nucleotide sequence. RAD4 encodes a somewhat basic protein of 754 amino acids (aa) with an Mr of 87173. RAD4 contains several groups of 4-7 consecutive basic aa residues that could be involved in DNA binding and it also contains an α-helix-turn-α-helix motif for DNA binding. Like several other DNA repair proteins of S. cerevisiae, the C terminus of RAD4 protein is highly acidic.

AB - The RAD4 gene of Saccharomyces cerevisiae is required for the incision step of excision repair. We have cloned the RAD4 gene and determined its nucleotide sequence. RAD4 encodes a somewhat basic protein of 754 amino acids (aa) with an Mr of 87173. RAD4 contains several groups of 4-7 consecutive basic aa residues that could be involved in DNA binding and it also contains an α-helix-turn-α-helix motif for DNA binding. Like several other DNA repair proteins of S. cerevisiae, the C terminus of RAD4 protein is highly acidic.

KW - DNA binding

KW - E. coli

KW - Recombinant DNA

KW - gap repair

KW - histone binding

KW - incision

KW - toxic

UR - https://www.scopus.com/pages/publications/0024238771

UR - https://www.scopus.com/pages/publications/0024238771#tab=citedBy

U2 - 10.1016/0378-1119(88)90186-2

DO - 10.1016/0378-1119(88)90186-2

M3 - Article

C2 - 3073107

AN - SCOPUS:0024238771

SN - 0378-1119

VL - 74

SP - 535

EP - 541

JO - Gene

JF - Gene

IS - 2

ER -

Cloning and nucleotide sequence analysis of the Saccharomyces cerevisiae RAD4 gene required for excision repair of UV-damaged DNA

Abstract

Keywords

ASJC Scopus subject areas

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