Cloning and sequence analysis of genomic fragment encoding 190KD protein antigen of HL-93 spotted fever group Rickettsiae

Jianzhi Zhang, Mingyuan Fan, Dezeng Bi

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Genomic fragment encoding 190kD protein antigen was amplified from spotted fever group (SFG) rickettsiae HL-93 strain by polymerase chain reaction with a pair of primers Rr 190. 70p and Rr 190. 602n which were designed from DNA sequences encoding 190kD protein antigen of R. rickettsii and cloned into E. coli JM101 by using plasmid vector PGEM-T. The genomic fragment was sequenced and compared with that of R. rickettsii and R. japonica which were reported previously. The results showed that 31 and 24 nucleotides in HL-93 strain differed from that in R. rickettsii and R. japonica, respectively. The genetic homology was 94% and 95. 5% respectively, and amino acid homology was 87% and 89% respectively. All data suggest that HL-93 strain is a new member of SFG rickettsiae.

Original languageEnglish (US)
Pages (from-to)227-230
Number of pages4
JournalChinese Journal of Microbiology and Immunology
Volume16
Issue number3
StatePublished - 1996
Externally publishedYes

Fingerprint

Rickettsia
Sequence Analysis
Organism Cloning
Fever
Antigens
Proteins
Plasmids
Nucleotides
Escherichia coli
Amino Acids
Polymerase Chain Reaction

Keywords

  • Clone
  • DNA sequence
  • Spotted fever group rickettsiae

ASJC Scopus subject areas

  • Immunology and Microbiology(all)
  • Microbiology (medical)

Cite this

Cloning and sequence analysis of genomic fragment encoding 190KD protein antigen of HL-93 spotted fever group Rickettsiae. / Zhang, Jianzhi; Fan, Mingyuan; Bi, Dezeng.

In: Chinese Journal of Microbiology and Immunology, Vol. 16, No. 3, 1996, p. 227-230.

Research output: Contribution to journalArticle

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